LOCUS       sequence-110441-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic
            loci using the Crispr/Cas technology. Can be converted by cassette 
            exchange into other Trojan exons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pT-GEM(1)
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    52..151
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     protein_bind    158..191
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    198..332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(222..241)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     276..295
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(604..624)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          754..819
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 ccgcattacc ctcactaaag ggaacctccc cactagtggt accggcgcgc ccccaggtca
       61 gaagcggttt tcgggagtag tgccccaact ggggtaacct ttgagttctc tcagttgggg
      121 gcgtagggtc gccgacatga cacaaggggt tctcgagata acttcgtata atgtatgcta
      181 tacgaagtta tgctagcgat ccagacatga taagatacat tgatgagttt ggacaaacca
      241 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      301 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
      361 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
      421 gtatggctga ttatgatctc tagtcaaggc actatacatc aaatattttt ccataatttt
      481 cttgtatagc agtgcagctt tttcctttgt ggtgtaaata gcaaagcaag caagagttct
      541 attactaaac acagcatgac tcaaaaaact tagcaattct gaaggaaagt ccttggggtc
      601 ttctaccttt ctcttctttt ttggaggagt agaatgttga gagtcagcag tagcctcatc
      661 atcactagat ggcatttctt ctgagcaaaa caggttttcc tcattaaagg cattccacca
      721 ctgctcccat tcatcagttc cataggttgg aatctaaaat acacaaacaa ttagaatcag
      781 tagtttaaca cattatacac ttaaaaattt tatatttacc ttagagcttt aaatctctgt
      841 aggtagtttg tccaattatg tcacaccaca gaagtaagg
//
