LOCUS       sequence-110448-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 
            standard parts for Golden Gate cloning. Parts can be released with a
            user-defined 4-base-pair, 5 prime overhangs using BsaI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Patron NJ, Orzaez D, Marillonnet S, Warzecha H, Matthewman C, Youles
            M, Raitskin O, Leveau A, Farre G, Rogers C, Smith A, Hibberd J, Webb
            AA, Locke J, Schornack S, Ajioka J, Baulcombe DC, Zipfel C, Kamoun 
            S, Jones JD, Kuhn H, Robatzek S, Van Esse HP, Sanders D, Oldroyd G, 
            Martin C, Field R, O'Connor S, Fox S, Wulff B, Miller B, Breakspear 
            A, Radhakrishnan G, Delaux PM, Loque D, Granell A, Tissier A, Shih 
            P, Brutnell TP, Quick WP, Rischer H, Fraser PD, Aharoni A, Raines C,
            South PF, Ane JM, Hamberger BR, Langdale J, Stougaard J, Bouwmeester
            H, Udvardi M, Murray JA, Ntoukakis V, Schafer P, Denby K, Edwards 
            KJ, Osbourn A, Haseloff J
  TITLE     Standards for plant synthetic biology: a common syntax for exchange 
            of DNA parts.
  JOURNAL   New Phytol. 2015 Jul 14. doi: 10.1111/nph.13532.
  PUBMED    26171760
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol.
            2015 Jul 14. doi: 10.1111/nph.13532."
COMMENT     Sequence Label: pUAP1
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     190..208
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(212..255)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     protein_bind    379..400
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(415..420,421..438,439..445)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    453..469
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             487..498
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
       61 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
      121 cgtgcccgat caactcgagt gccacctgac gtctaagaaa ccattattat catgacatta
      181 acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc
      241 gctaaggatg atttctggaa ttcggtctca ttgtcttcca atacgcaaac cgcctctccc
      301 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
      361 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
      421 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacata
      481 ctagagaaag aggagaaata ctagatggct tcctccgagg acgttatcaa agagttcatg
      541 cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa
      601 ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt
      661 ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac
      721 gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa
      781 tgggaacgtg ttatgaactt cgaggacggt ggtgttgtta ccgttaccca ggactcctcc
      841 ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtacc
//
