LOCUS       sequence-110451-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 
            standard parts for Golden Gate cloning. Parts can be released with a
            user-defined 4-base-pair, 5 prime overhangs using BsaI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Patron NJ, Orzaez D, Marillonnet S, Warzecha H, Matthewman C, Youles
            M, Raitskin O, Leveau A, Farre G, Rogers C, Smith A, Hibberd J, Webb
            AA, Locke J, Schornack S, Ajioka J, Baulcombe DC, Zipfel C, Kamoun 
            S, Jones JD, Kuhn H, Robatzek S, Van Esse HP, Sanders D, Oldroyd G, 
            Martin C, Field R, O'Connor S, Fox S, Wulff B, Miller B, Breakspear 
            A, Radhakrishnan G, Delaux PM, Loque D, Granell A, Tissier A, Shih 
            P, Brutnell TP, Quick WP, Rischer H, Fraser PD, Aharoni A, Raines C,
            South PF, Ane JM, Hamberger BR, Langdale J, Stougaard J, Bouwmeester
            H, Udvardi M, Murray JA, Ntoukakis V, Schafer P, Denby K, Edwards 
            KJ, Osbourn A, Haseloff J
  TITLE     Standards for plant synthetic biology: a common syntax for exchange 
            of DNA parts.
  JOURNAL   New Phytol. 2015 Jul 14. doi: 10.1111/nph.13532.
  PUBMED    26171760
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol.
            2015 Jul 14. doi: 10.1111/nph.13532."
COMMENT     Sequence Label: pUAP1
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      144..215
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      231..258
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      286..343
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(367..384)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(618..637)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaaaccgaca tcaaactgga catcacctcc cacaacgagg actacaccat cgttgaacag
       61 tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt
      121 agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      181 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
      241 cgggtgggcc tttctgcgtt tatagaagac aacgagaccc tgcagtccgg caaaaaaggg
      301 caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttatgca
      361 ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      421 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      481 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      541 ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      601 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      661 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      721 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      781 gctgggctgt gtgcacgaac cccc
//
