LOCUS       sequence-110453-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of St1 dCas9-3XGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pHAGE-TO-nls-st1dCas9-3nls-3XGFP
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(46..759)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 aattttgtaa tccagaggtt gattatctag attaccctac tcgagtttgt acagttcatc
       61 cataccatgc gtgatgcccg ctgcggttac gaactccagc agaaccatat gatcgcgttt
      121 ctcgttcgga tctttagaca gaacgctttg cgtgctcaga tagtgattgt ctggcagcag
      181 aacaggacca tcaccgattg gagtgttttg ctggtagtga tcagccagct gcacgctgcc
      241 atcctccacg ttgtggcgaa ttttaaaatt cgctttaatg ccattttttt gtttatcggc
      301 ggtgatgtaa acattgtggc tgttaaaatt gtattccagc ttatggccca ggatattgcc
      361 gtcttcttta aagtcaatgc ctttcagctc aatgcggttt accagggtat cgccttcaaa
      421 tttcacttcc gcacgcgttt tgtacgtgcc gtcatcctta aaggaaatcg tgcgttcctg
      481 cacatagcct tccggcatgg cggacttgaa gaagtcatgc tgcttcatat ggtccggata
      541 acgagcaaag cactgaacac cataagtcag cgtcgttacc agagtcggcc aaggtaccgg
      601 cagtttacca gtagtacaga tgaacttcag cgtcagttta ccattagttg cgtcaccttc
      661 accctcgcca cgcacggaaa acttatgacc gttgacatca ccatccagtt ccaccagaat
      721 agggacgaca ccagtgaaca gctcttcgcc tttacgcatg ctaccatcga tgctaccttt
      781 gtacagttca tccataccat gcgtgatgcc cgctgcggtt acgaactcca gcagaaccat
      841 atgatcgcgt ttctcgttcg gatctttaga cagaacgctt tgcgtgctca gatagtgatt
      901 gtctggcagc agaac
//
