LOCUS       sequence-110455-         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recipient overexpressing vector to construct FLAG tagged fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Yanez-Carrillo P, Orta-Zavalza E, Gutierrez-Escobedo G, 
            Patron-Soberano A, De Las Penas A, Castano I
  TITLE     Expression vectors for C-terminal fusions with fluorescent proteins 
            and epitope tags in Candida glabrata.
  JOURNAL   Fungal Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020.
  PUBMED    25986172
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020."
COMMENT     Sequence Label: pYC119
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        35..433
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     437..453
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     437..453
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             463..486
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             493..516
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             523..546
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(590..606)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(591..607)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 ttaaccctca ctaaagggaa caaaagctgg agctcatagc ttcaaaatgt ttctactcct
       61 tttttactct tccagatttt ctcggactcc gcgcatcgcc gtaccacttc aaaacaccca
      121 agcacagcat actaaatttc ccctctttct tcctctaggg tgtcgttaat tacccgtact
      181 aaaggtttgg aaaagaaaaa agagaccgcc tcgtttcttt ttcttcgtcg aaaaaggcaa
      241 taaaaatttt tatcacgttt ctttttcttg aaaatttttt ttttgatttt tttctctttc
      301 gatgacctcc cattgatatt taagttaata aacggtcttc aatttctcaa gtttcagttt
      361 catttttctt gttctattac aacttttttt acttcttgct cattagaaag aaagcatagc
      421 aatctaatct aagttttcta gaactagtgg atcctaggag cagactacaa agatgacgat
      481 gacaaaggag ccgattataa ggacgatgac gataaagggg ctgactacaa agatgacgat
      541 gacaagtgat aagatccccc gggctgcagg aattcgatat caagcttatc gataccgtcg
      601 acctcgagaa cacaacccac agctaccacc atcaacaata tttatatata taacgtacac
      661 atagaaatca cacaaacaga gtatttattc ttaactacat gaactaccat cagaccgtct
      721 ggg
//
