LOCUS       sequence-110456-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for expression of St1 sgRNA3.1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pLH-stsgRNA3.1
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    110..227
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(395..700)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(497..516)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 agagcagccg cgtccctgcg caaacccagg gctgccttgg aaaaggcgca accccaaccc
       61 cctcgagccg cggccaaagt ggatctctgc tgtccctgta ataaacccga aaattttgaa
      121 tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta
      181 ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaattg tggatgaata
      241 ctgccatttg tctcgaggtc gagaattcaa aaaaacaccc tgccatagaa tgacagggtg
      301 ttgatttcgg catgaagcct tatctttgta gcttctgcaa gatttaattt cttaaatctt
      361 gagagtacaa agactggtct tcgtcctgca gggtttatat tccccagaac atcaggttaa
      421 tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc ccgataacgg
      481 agaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc ccgatatgca
      541 ccaccgggta aagttcacgg gagactttat ctgacagcag acgtgcactg gccaggggga
      601 tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca aacagacgat
      661 aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagtc cctgttctcg
      721 tcagcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc ttcctgattt
      781 tccgctttcc agcgttcggc acgcagac
//
