LOCUS       sequence-110457-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Sp dCas9-GFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pHAGE-EFS-dCas9-GFP
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(72..785)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(798..818)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttgtaatcca gaggttgatt atctagatta ccctactcga ggctcccatc gatgccagag
       61 cggccgccag atttgtacag ttcatccata ccatgcgtga tgcccgctgc ggttacgaac
      121 tccagcagaa ccatatgatc gcgtttctcg ttcggatctt tagacagaac gctttgcgtg
      181 ctcagatagt gattgtctgg cagcagaaca ggaccatcac cgattggagt gttttgctgg
      241 tagtgatcag ccagctgcac gctgccatcc tccacgttgt ggcgaatttt aaaattcgct
      301 ttaatgccat ttttttgttt atcggcggtg atgtaaacat tgtggctgtt aaaattgtat
      361 tccagcttat ggcccaggat attgccgtct tctttaaagt caatgccttt cagctcaatg
      421 cggtttacca gggtatcgcc ttcaaatttc acttccgcac gcgttttgta cgtgccgtca
      481 tccttaaagg aaatcgtgcg ttcctgcaca tagccttccg gcatggcgga cttgaagaag
      541 tcatgctgct tcatatggtc cggataacga gcaaagcact gaacaccata agtcagcgtc
      601 gttaccagag tcggccaagg taccggcagt ttaccagtag tacagatgaa cttcagcgtc
      661 agtttaccat tagttgcgtc accttcaccc tcgccacgca cggaaaactt atgaccgttg
      721 acatcaccat ccagttccac cagaataggg acgacaccag tgaacagctc ttcgccttta
      781 cgcatacttc cggatcctac cttcctcttc ttcttgggag agccactagt agagccgtct
      841 ccaccgagct gagagaggtc gattctt
//
