LOCUS       sequence-110523-         627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recipient replicative vector to construct HA tagged fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Yanez-Carrillo P, Orta-Zavalza E, Gutierrez-Escobedo G, 
            Patron-Soberano A, De Las Penas A, Castano I
  TITLE     Expression vectors for C-terminal fusions with fluorescent proteins 
            and epitope tags in Candida glabrata.
  JOURNAL   Fungal Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020.
  PUBMED    25986172
REFERENCE   2  (bases 1 to 627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020."
COMMENT     Sequence Label: pYC62
FEATURES             Location/Qualifiers
     source          1..627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(92..181)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     primer_bind     complement(222..238)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(223..239)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      complement(260..507)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     473..491
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 ttaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc gctctagaaa
       61 gtataggaac ttcggatctt cagcactgag cagcgtaatc tggaacgtca tatggatagg
      121 agcctgcata gtccgggacg tcatacggat agcccgcata gtcaggaaca tcgtatgggt
      181 ataggatccc ccgggctgca ggaattcgat atcaagctta tcgataccgt cgacctcgag
      241 ggggggcccg gtaccggccg caaattaaag ccttcgagcg tcccaaaacc ttctcaagca
      301 aggttttcag tataatgtta catgcgtaca cgcgtctgta cagaaaaaaa agaaaaattt
      361 gaaatataaa taacgttctt aatactaaca taactataaa aaaataaata gggacctaga
      421 cttcaggttg tctaactcct tccttttcgg ttagagcgga tgtgggggga gggcgtgaat
      481 gtaagcgtga cataactaat tacatgactc gacttagggg cagggcatgc tcatgtagag
      541 cgcctgctcg ccgtccgagg cggtgccgtc gtacagggcg gtgtccaggc cgcagagggt
      601 gaaccccatc cgccggtacg cgtggat
//
