LOCUS       sequence-110525-         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Blelloch R, Venere M, Yen J, Ramalho-Santos M
  TITLE     Generation of induced pluripotent stem cells in the absence of drug 
            selection.
  JOURNAL   Cell Stem Cell. 2007 Sep 13;1(3):245-7. doi: 
            10.1016/j.stem.2007.08.008. Epub 2007 Sep 6.
  PUBMED    18371358
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2007.08.008"; journalName: "Cell Stem Cell"; date: 
            "2007-09-13- 13"; volume: "1"; issue: "3"; pages: "245-7"
COMMENT     Sequence Label: pLOVE empty vector
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(107..231)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             284..325
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(363..396)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(505..525)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
       61 atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat
      121 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
      181 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg ttgatatcca
      241 gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa
      301 ccctctcctc ggtctcgatt ctacgcgtac cggttagtaa tgacaattcg tcgagggacc
      361 taataacttc gtatagcata cattatacga agttatacat gtttaagggt tccggttcca
      421 ctaggtacaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
      481 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
      541 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
      601 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
      661 gtgcactgtg tttgctgacg caacccccac tggttgg
//
