LOCUS       sequence-110529-         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express codon optimized Lin-28 and wild type Nanog. A single 
            plasmid reprogramming system using a mini-intronic plasmid (MIP)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Diecke S, Lu J, Lee J, Termglinchan V, Kooreman NG, Burridge PW, 
            Ebert AD, Churko JM, Sharma A, Kay MA, Wu JC
  TITLE     Novel codon-optimized mini-intronic plasmid for efficient, 
            inexpensive, and xeno-free induction of pluripotency.
  JOURNAL   Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081.
  PUBMED    25628230
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-01-28"; volume: "5:8081. doi"; pages: " 
            10.1038/srep08081"
COMMENT     Sequence Label: MIP 339 CoMiP coLin28 2A Nanog IRES eGFP
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      482..491
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
       61 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
      121 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      181 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactcctgca
      241 aaccacgttg tggtagaatt ggtaaagaga gtcgtgtaaa atatcgagtt cgcacatctt
      301 gttgtctgat tattgatttt tggcgaaacc atttgatcat atgacaagat gtgtatctac
      361 cttaacttaa tgattttgat aaaaatcatt aggtaccccg gcccgcactg acccctggtg
      421 ttgctttttt tttttaggcc gcaagctgaa gcgtgtccac tagctgacta gctaaccgcg
      481 ggccaccatg ggcagcgtgt ccaaccagca gtttgccggc ggatgcgcca aggccgccga
      541 ggaagctcct gaagaggccc ccgaagatgc cgctagagcc gccgatgaac cccagctgct
      601 gcatggcgcc ggaatctgca agtggttcaa cgtgcggatg ggcttcggct tcctgagcat
      661 gacagccaga gccggcgtgg ccctggaccc ccctgtggat gtgttcgtgc accagagcaa
      721 gctgcacatg gaaggcttca gatccctg
//
