LOCUS       sequence-110531-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Nm dCas9-3XGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pHAGE-TO-nmdCas9-3XGFP
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..749)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 tccagaggtt gattatctag attaccctac tcgagtttgt acagttcatc cataccatgc
       61 gtgatgcccg ctgcggttac gaactccagc agaaccatat gatcgcgttt ctcgttcgga
      121 tctttagaca gaacgctttg cgtgctcaga tagtgattgt ctggcagcag aacaggacca
      181 tcaccgattg gagtgttttg ctggtagtga tcagccagct gcacgctgcc atcctccacg
      241 ttgtggcgaa ttttaaaatt cgctttaatg ccattttttt gtttatcggc ggtgatgtaa
      301 acattgtggc tgttaaaatt gtattccagc ttatggccca ggatattgcc gtcttcttta
      361 aagtcaatgc ctttcagctc aatgcggttt accagggtat cgccttcaaa tttcacttcc
      421 gcacgcgttt tgtacgtgcc gtcatcctta aaggaaatcg tgcgttcctg cacatagcct
      481 tccggcatgg cggacttgaa gaagtcatgc tgcttcatat ggtccggata acgagcaaag
      541 cactgaacac cataagtcag cgtcgttacc agagtcggcc aaggtaccgg cagtttacca
      601 gtagtacaga tgaacttcag cgtcagttta ccattagttg cgtcaccttc accctcgcca
      661 cgcacggaaa acttatgacc gttgacatca ccatccagtt ccaccagaat agggacgaca
      721 ccagtgaaca gctcttcgcc tttacgcatg ctaccatcga tgctaccttt gtacagttca
      781 tccataccat gcgtgatgcc cgctgcggtt acgaactcca gcagaaccat atgatcgcgt
      841 ttctcgttcg gatctt
//
