LOCUS       sequence-110534-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Nm dCas9-3XmCherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pHAGE-TO-nmdCas9-3XmCherry
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..735)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(303..322)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     572..590
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ttgattatct agattaccct actcgagctt gtacagctcg tccatgccgc cggtggagtg
       61 gcggccctca gcgcgttcgt actgttccac gatggtgtag tcctcgttgt gggaggtgat
      121 gtccaacttg atgttgacgt tgtaggcgcc gggcagctgc acgggcttct tggccttgta
      181 ggtggtcttg acctcagcgt cgtagtggcc gccgtccttc agcttcagcc tctgcttgat
      241 ctcgcccttc agggcgccgt cctcggggta catccgctcg gaggaggcct cccagcccat
      301 ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg gtgccgcgca gcttcacctt
      361 gtagatgaac tcgccgtcct gcagtgagga gtcctgggtc acggtcacca cgccgccgtc
      421 ctcgaagttc atcacgcgct cccacttgaa gccctcgggg aaggacagct tcaagtagtc
      481 ggggatgtcg gcggggtgct tcacgtaggc cttggagccg tacatgaact gaggggacag
      541 gatgtcccag gcgaagggca gggggccacc cttggtcacc ttcagcttgg cggtctgggt
      601 gccctcgtag gggcggccct cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga
      661 gccctccatg tgcaccttga agcgcatgaa ctccttgatg atggccatgt tatcctcctc
      721 gcccttgctc accatgctac catcgatgct acccttgtac agctcgtcca tgccgccggt
      781 ggagtggcgg ccctcagcgc gttcgtactg ttccacgatg gtgtagtcct cgttgtggga
      841 ggtgatgtcc aacttgatgt tgacgttgta ggcgccgggc agctgcacgg gcttcttggc
      901 cttgtaggtg gtcttgacct
//
