LOCUS       sequence-110535-         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: Hras (G12V)-pcw107
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(84..116,117..119,120..653)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     protein_bind    complement(655..679)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcaccgacct ctctccccag gggctagcat cgatggatca acaagttttg tacaaaaaag
       61 ttggcagtag cggcggcggc accatgacgg aatataagct ggtggtggtg ggcgccgtcg
      121 gtgtgggcaa gagtgcgctg accatccagc tgatccagaa ccattttgtg gacgaatacg
      181 accccactat agaggattcc taccggaagc aggtggtcat tgatggggag acgtgcctgt
      241 tggacatcct ggataccgcc ggccaggagg agtacagcgc catgcgggac cagtacatgc
      301 gcaccgggga gggcttcctg tgtgtgtttg ccatcaacaa caccaagtct tttgaggaca
      361 tccaccagta cagggagcag atcaaacggg tgaaggactc ggatgacgtg cccatggtgc
      421 tggtggggaa caagtgtgac ctggctgcac gcactgtgga atctcggcag gctcaggacc
      481 tcgcccgaag ctacggcatc ccctacatcg agacctcggc caagacccgg cagggagtgg
      541 aggatgcctt ctacacgttg gtgcgtgaga tccggcagca caagctgcgg aagctgaacc
      601 ctcctgatga gagtggcccc ggctgcatga gctgcaagtg tgtgctctcc tgattgccaa
      661 ctttcttgta caaagtggtt accggttagt aatgaacgcg ttaagtcgac aatcaacctc
      721 tggattac
//
