LOCUS       sequence-110537-         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: Hras (G12V)-pcw107
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    50..74
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(76..609,610..612,613..645))
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     protein_bind    complement(663..687)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtaatccaga ggttgattgt cgacttaacg cgttcattac taaccggtaa ccactttgta
       61 caagaaagtt ggcaatcagg agagcacaca cttgcagctc atgcagccgg ggccactctc
      121 atcaggaggg ttcagcttcc gcagcttgtg ctgccggatc tcacgcacca acgtgtagaa
      181 ggcatcctcc actccctgcc gggtcttggc cgaggtctcg atgtagggga tgccgtagct
      241 tcgggcgagg tcctgagcct gccgagattc cacagtgcgt gcagccaggt cacacttgtt
      301 ccccaccagc accatgggca cgtcatccga gtccttcacc cgtttgatct gctccctgta
      361 ctggtggatg tcctcaaaag acttggtgtt gttgatggca aacacacaca ggaagccctc
      421 cccggtgcgc atgtactggt cccgcatggc gctgtactcc tcctggccgg cggtatccag
      481 gatgtccaac aggcacgtct ccccatcaat gaccacctgc ttccggtagg aatcctctat
      541 agtggggtcg tattcgtcca caaaatggtt ctggatcagc tggatggtca gcgcactctt
      601 gcccacaccg acggcgccca ccaccaccag cttatattcc gtcatggtgc cgccgccgct
      661 actgccaact tttttgtaca aacttgttga tccatcgatg ctagcccct
//
