LOCUS       sequence-110538-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for expression of Sp sgRNA2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pLH-spsgRNA2
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    100..217
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(372..677)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(474..493)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 cgtccctgcg caaacccagg gctgccttgg aaaaggcgca accccaaccc cctcgagccg
       61 cggccaaagt ggatctctgc tgtccctgta ataaacccga aaattttgaa tttttgtaat
      121 ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc
      181 tgcactgtac cccccaatcc ccccttttct tttaaaattg tggatgaata ctgccatttg
      241 tctcgaggtc gagaattcaa aaaaagcacc gactcggtgc cactttttca agttgataac
      301 ggactagcct tatttaaact tgctatgctg tttccagcat agctcttaaa cgggtcttcg
      361 tcctgcaggg tttatattcc ccagaacatc aggttaatgg cgtttttgat gtcattttcg
      421 cggtggctga gatcagccac ttcttccccg ataacggaga ccggcacact ggccatatcg
      481 gtggtcatca tgcgccagct ttcatccccg atatgcacca ccgggtaaag ttcacgggag
      541 actttatctg acagcagacg tgcactggcc agggggatca ccatccgtcg cccgggcgtg
      601 tcaataatat cactctgtac atccacaaac agacgataac ggctctctct tttataggtg
      661 taaaccttaa actgcatttc accagtccct gttctcgtca gcaaaagagc cgttcatttc
      721 aataaaccgg gcgacctcag ccatcccttc ctgattttcc gctttccagc gttcggcacg
      781 cagacgacgg gcttcatt
//
