LOCUS       sequence-110541-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for expression of St1 sgRNA2.1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pLH-stsgRNA2.1
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    105..222
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(380..685)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(482..501)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 agccgcgtcc ctgcgcaaac ccagggctgc cttggaaaag gcgcaacccc aaccccctcg
       61 agccgcggcc aaagtggatc tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt
      121 gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc tactattctt
      181 tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat gaatactgcc
      241 atttgtctcg aggtcgagaa ttcaaaaaaa caccctgcca tagaatgaca gggtgttgat
      301 ttcggcatga agccttatct ttgtagcttc tgcaagattt ctcttgagag tacaaagact
      361 ggtcttcgtc ctgcagggtt tatattcccc agaacatcag gttaatggcg tttttgatgt
      421 cattttcgcg gtggctgaga tcagccactt cttccccgat aacggagacc ggcacactgg
      481 ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt
      541 cacgggagac tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc
      601 cgggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt
      661 tataggtgta aaccttaaac tgcatttcac cagtccctgt tctcgtcagc aaaagagccg
      721 ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt
      781 tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccagacc ggagatattg
      841 acatcatata tgccttgagc aactgatagc tgtcgctgtc aactgtcact gtaatacgct
      901 gcttcatagc acacctc
//
