LOCUS       sequence-110543-         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral vector for knocking down beta-catenin gene 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Onder TT, Gupta PB, Mani SA, Yang J, Lander ES, Weinberg RA
  TITLE     Loss of E-cadherin promotes metastasis via multiple downstream 
            transcriptional pathways.
  JOURNAL   Cancer Res. 2008 May 15. 68(10):3645-54.
  PUBMED    18483246
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2008 May 15. 68(10):3645-54."
COMMENT     Sequence Label: pLKO.1 puro shRNA beta-catenin
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    139..256
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        305..815
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     734..752
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(837..856)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgggcttgg aatgagactg
       61 ctgatctcga gatcagcagt ctcattccaa gctttttgaa ttctcgacct cgagacaaat
      121 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
      181 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      241 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggccgcggct
      301 cgagggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct
      361 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca
      421 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc
      481 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac
      541 aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg
      601 cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga
      661 gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt
      721 tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct
      781 ccctcgttga ccgaatcacc gacctctctc cccaggggga tccaccggag cttaccatga
      841 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
      901 ccctcgccgc cgcgttcgcc ga
//
