LOCUS       sequence-110545-         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: myr-FLAG-PIK3CA-pcw107
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    42..66
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             84..125
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             150..173
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tcaccgacct ctctccccag ggggctagca tcgatggatc aacaagtttg tacaaaaaag
       61 ttggcagggg cggcggcggc accatggggt cttcaaaatc taaaccaaag gaccccagcc
      121 agcgccggcg caggatccga ggttaccttg actacaaaga cgatgacgac aagcaattga
      181 gcgacgagat ccgaacaatg cctccaagac catcatcagg tgaactgtgg ggcatccact
      241 tgatgccccc aagaatccta gtagaatgtt tactaccaaa tgggatgata gtgactttag
      301 aatgcctccg tgaggctacg ttaataacga taaagcatga actatttaaa gaagcaagaa
      361 aataccctct ccatcaactt cttcaagatg aatcttctta cattttcgta agtgttaccc
      421 aagaagcaga aagggaagaa ttttttgatg aaacaagacg actttgtgac cttcggcttt
      481 ttcaaccctt tttaaaagta attgaaccag taggcaaccg tgaagaaaag atcctcaatc
      541 gagaaattgg ttttgctatc ggcatgccag tgtgtgaatt cgatatggtt aaagatccag
      601 aagtacagga cttccgaaga aatattctca atgtttgtaa agaagctgtg gatcttaggg
      661 atcttaattc acctcatagt agagcaatgt atg
//
