LOCUS       sequence-110546-         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Singlet donor (phase 1) for in vivo RMCE with MiMIC inserts to make 
            Gal4 driver..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pC-(loxP2-attB2-SA(1)-T2A-Gal4-Hsp70)
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..51
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    167..236
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             405..458
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tcgggccccc cctcgagata acttcgtata atgtatgcta tacgaagtta tcaattggct
       61 tattgggatt tcaatttaag atgctgctaa ataaagttag tcacttacgg actggggagg
      121 caccccgtca tcactcgcca gaaatgcaat tgatgtaggt cacggtctcg aagccgcggt
      181 gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccacctca cccatctggt
      241 ccatcatgat ggtcgactct agaagtcgat ccaacatggc gacttgtccc atccccggca
      301 tgtttaaata tactaattat tcttgaacta attttaatca accgatttat ctctcttccg
      361 caggtggcgg aggttccggt ggaagcggag gtagcggcgg atccgagggc cgcggcagcc
      421 tgctgacctg cggcgatgtg gaggagaacc ccgggcccat gaagctgctg agcagcatcg
      481 agcaggcctg cgatatctgc cgcctgaaga agctgaagtg cagcaaggag aagcccaagt
      541 gcgccaagtg cctgaagaac aactgggagt gccgctacag ccccaagacc aagcgcagcc
      601 ccctgacccg cgcccacctg accgaggtgg agagccgcct ggagcgcctg gagcagctgt
      661 tcctgctgat cttcccccgc gaggatctg
//
