LOCUS       sequence-110561-       11234 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  reporter plasmids used in YeastFab work, in which promoters could be
            inserted just upstream YFP(YEVenus) to drive its expression, also 
            contains an mCherry gene driven by TEF2 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11234)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 11234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: pYG026
FEATURES             Location/Qualifiers
     source          1..11234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(512..528)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(512..528)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(565..585)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(565..583)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(604..620)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(604..620)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(617..639)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    628..644
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(652..658,659..676,677..682))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(835..852)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1006..1594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1086..1105)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1765..2556,2557..2625))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2388..2407
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2626..2730)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    2767..3270
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     3312..3330
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3368..3390)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3490..3509
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(4002..5096)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(5109..5513)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(5813..6268)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5950..5971)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6162..6181
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6398..6420
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6412..6429
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6413..6429
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6439..6458
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6439..6457
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     6482..6498
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6483..6499
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(7071..7258)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(7279..7995)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
     CDS             complement(8054..8359)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8156..8175)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     promoter        8716..8930
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             8931..9734
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      complement(9743..9930)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(9957..10667)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(10235..10254)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     10504..10522
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctttaaagta tagatagaat tgattgctgc ttatgaggat atggatttag agctaatagt
       61 tgtaatagaa ataaaagcca tttagaatag gaattgaata caattttatg agctttaaat
      121 atttaatgat gaatagtgcg tttagaacgc ttcatcatgc ttcaacaagc ttctgcaaac
      181 ctcatttgag tagcaatagc tgtttacttt tttccgcgac tttattcgaa gttatggtga
      241 acttttcgtg gtttcacacc taataacgcc cagcggtagt acaattcaaa gtagtaggta
      301 ccaatggtag tactagtggg tgatagtagc aaatttctct atgaaaatga attgttgctc
      361 tgcaatgatg ttaatgtgga catgacatga attttcgcga tatgttgatg taaataataa
      421 catgattttc gtggatcatt tcgtaaacca taggtttatt tcgtgaatta ctaagagaaa
      481 ggcatagaaa caggacttgc gggatacggg ggatccacta gttctagagc ggccgccacc
      541 gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
      601 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatagg
      661 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgaggtaac tcacattaat
      721 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
      781 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
      841 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      901 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      961 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1021 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1081 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1141 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1201 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1261 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1321 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1381 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1441 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1501 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1561 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1621 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     1681 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1741 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     1801 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     1861 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagatc cacgctcacc
     1921 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     1981 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     2041 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     2101 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     2161 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     2221 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     2281 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     2341 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     2401 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     2461 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     2521 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     2581 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     2641 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     2701 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc
     2761 cttttcatca cgtgctataa aaataattat aatttaaatt ttttaatata aatatataaa
     2821 ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt tccgaagatg
     2881 taaaagactc tagggggatc gccaacaaat actacctttt atcttgctct tcctgctctc
     2941 aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg
     3001 tgattttaca ttttacttat cgttaatcga atgtatatct atttaatctg cttttcttgt
     3061 ctaataaata tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct ttgtttattt
     3121 ttttttcttc attccgtaac tcttctacct tctttattta ctttctaaaa tccaaataca
     3181 aaacataaaa ataaataaac acagagtaaa ttcccaaatt attccatcat taaaagatac
     3241 gaggcgcgtg taagttacag gcaagcgatc cgtcctaaga aaccattatt atcatgacat
     3301 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     3361 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     3421 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     3481 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatcgac tacgtcgtaa
     3541 ggccgtttct gacagagtaa aattcttgag ggaactttca ccattatggg aaatggttca
     3601 agaaggtatt gacttaaact ccatcaaatg gtcaggtcat tgagtgtttt ttatttgttg
     3661 tatttttttt tttttagaga aaatcctcca atatcaaatt aggaatcgta gtttcatgat
     3721 tttctgttac acctaacttt ttgtgtggtg ccctcctcct tgtcaatatt aatgttaaag
     3781 tgcaattctt tttccttatc acgttgagcc attagtatca atttgcttac ctgtattcct
     3841 ttactatcct cctttttctc cttcttgata aatgtatgta gattgcgtat atagtttcgt
     3901 ctaccctatg aacatattcc attttgtaat ttcgtgtcgt ttctattatg aatttcattt
     3961 ataaagttta tgtacaaata tcataaaaaa agagaatctt tttaagcaag gattttctta
     4021 acttcttcgg cgacagcatc accgacttcg gtggtactgt tggaaccacc taaatcacca
     4081 gttctgatac ctgcatccaa aaccttttta actgcatctt caatggcctt accttcttca
     4141 ggcaagttca atgacaattt caacatcatt gcagcagaca agatagtggc gatagggtca
     4201 accttattct ttggcaaatc tggagcagaa ccgtggcatg gttcgtacaa accaaatgcg
     4261 gtgttcttgt ctggcaaaga ggccaaggac gcagatggca acaaacccaa ggaacctggg
     4321 ataacggagg cttcatcgga gatgatatca ccaaacatgt tgctggtgat tataatacca
     4381 tttaggtggg ttgggttctt aactaggatc atggcggcag aatcaatcaa ttgatgttga
     4441 accttcaatg tagggaattc gttcttgatg gtttcctcca cagtttttct ccataatctt
     4501 gaagaggcca aaacattagc tttatccaag gaccaaatag gcaatggtgg ctcatgttgt
     4561 agggccatga aagcggccat tcttgtgatt ctttgcactt ctggaacggt gtattgttca
     4621 ctatcccaag cgacaccatc accatcgtct tcctttctct taccaaagta aatacctccc
     4681 actaattctc tgacaacaac gaagtcagta cctttagcaa attgtggctt gattggagat
     4741 aagtctaaaa gagagtcgga tgcaaagtta catggtctta agttggcgta caattgaagt
     4801 tctttacgga tttttagtaa accttgttca ggtctaacac taccggtacc ccatttagga
     4861 ccacccacag cacctaacaa aacggcatca accttcttgg aggcttccag cgcctcatct
     4921 ggaagtggga cacctgtagc atcgatagca gcaccaccaa ttaaatgatt ttcgaaatcg
     4981 aacttgacat tggaacgaac atcagaaata gctttaagaa ccttaatggc ttcggctgtg
     5041 atttcttgac caacgtggtc acctggcaaa acgacgatct tcttaggggc agacataggg
     5101 gcagacatta gaatggtata tccttgaaat atatatatat attgctgaaa tgtaaaaggt
     5161 aagaaaagtt agaaagtaag acgattgcta accacctatt ggaaaaaaca ataggtcctt
     5221 aaataatatt gtcaacttca agtattgtga tgcaagcatt tagtcatgaa cgcttctcta
     5281 ttctatatga aaagccggtt ccggcctctc acctttcctt tttctcccaa tttttcagtt
     5341 gaaaaaggta tatgcgtcag gcgacctctg aaattaacaa aaaatttcca gtcatcgaat
     5401 ttgattctgt gcgatagcgc ccctgtgtgt tctcgttatg ttgaggaaaa aaataatggt
     5461 tgctaagaga ttcgaactct tgcatcttac gatacctgag tattcccaca gttaactgcg
     5521 gtcaagatat ttcttgaatc aggcgcctta gaccgctcgg ccaaacaacc aattacttgt
     5581 tgagaaatag agtataatta tcctataaat ataacgtttt tgaacacaca tgaacaagga
     5641 agtacaggac aattgatttt gaagagaatg tggattttga tgtaattgtt gggattccat
     5701 ttttaataag gcaataatat taggtatgtg gatatactag aagttctcct cgaccgtcga
     5761 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta
     5821 aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     5881 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     5941 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     6001 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     6061 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     6121 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     6181 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     6241 gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt
     6301 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     6361 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     6421 acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccccc
     6481 ctcgaggtcg acggtatcga taagcttgat atcgaattcc tgcagcccgt atccctaact
     6541 tcaatgtcaa agttgaaata ttctccttta gagcgctcca tttcttctat gaagcgtttt
     6601 gcggcaaact caccttcaac tgtcattggg aatgtcttat gatggttttt tggaattatt
     6661 attatcctac catcaagcgt ctgacattgc tgcagatttc tccatctcac tttatatttg
     6721 gtggcatttc taccactttt ttccaacagt ggtttggtag ggaccctgac tgacaattta
     6781 tgacctgcag tacattgtaa tgcaagacgc tgataaactg ttctacgcct gggatctaac
     6841 ctaccaggtt caccttcaaa agctctgtgt ttggtttttt gctgtatatt atagattttc
     6901 tgatagccct gtgtgacatt tatgacgcgg gcagcggagc catctgcgca cataacgtaa
     6961 gagttagccg tgacgtttgc gatgtcttta atttcaccgt tagccatcag aatagtcgtg
     7021 ttttcagaaa gcatggcatg catattaccc tgttatccct agcggatctg ccggtagagg
     7081 tgtggtcaat aagagcgacc tcatactata cctgagaaag caacctgacc tacaggaaag
     7141 agttactcaa gaataagaat tttcgtttta aaacctaaga gtcactttaa aatttgtata
     7201 cacttatttt ttttataact tatttaataa taaaaatcat aaatcataag aaattcgctt
     7261 atttagaagt ggcgcgcctt atttgtacaa ttcatcaata ccatgggtaa taccagcagc
     7321 agtaacaaat tctaacaaga ccatgtgatc tctcttttcg tttggatctt tggataaggc
     7381 agattgatag gataagtaat ggttgtctgg taacaagact ggaccatcac caattggagt
     7441 attttgttga taatggtcag ctaattgaac accaccatct tcaatgttgt gtctaatttt
     7501 gaagttagct ttgataccat tcttttgttt gtcagcagtg atgtaaacat tgtgagagtt
     7561 atagttgtat tccaatttgt gacctaaaat gttaccatct tctttaaaat caataccttt
     7621 taattcgatt ctattaacta aggtatcacc ttcaaacttg acttcagctc tggtcttgta
     7681 gttaccgtca tctttgaaaa aaatagttct ttcttgaaca taaccttctg gcatggcaga
     7741 cttgaaaaag tcatgttgtt tcatatgatc tgggtatcta gcaaaacatt gcaaaccata
     7801 acctaaagta gtgactaagg ttggccatgg aactggcaat ttaccagtag tacaaatcaa
     7861 ttttaaggtc aatttaccgt aagtagcatc accttcacct tcaccggaga cagaaaattt
     7921 gtgaccatta acatcaccat ctaattcaac caaaattggg acaacaccag tgaataattc
     7981 ttcaccttta gacataaaaa ccatctgaga cccgtcgacc tgcagactgg ctgtgtataa
     8041 gggagcctga catttatatt ccccagaaca tcaggttaat ggcgtttttg atgtcatttt
     8101 cgcggtggct gagatcagcc acttcttccc cgataacgga gaccggcaca ctggccatat
     8161 cggtggtcat catgcgccag ctttcatccc cgatatgcac caccgggtaa agttcacggg
     8221 ggactttatc tgacagcaga cgtgcactgg ccagggggat caccatccgt cgcccgggcg
     8281 tgtcaataat atcactctgt acatccacaa acagacgata acggctctct cttttatagg
     8341 tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt cagcaaaaga gccgttcatt
     8401 tcaataaacc gggcgacctc agccatccct tcctgatttt ccgctttcca gcgttcggca
     8461 cgcagacgac gggcttcatt ctgcatggtt gtgcttacca gaccggagat attgacatca
     8521 tatatgcctt gagcaactga tagctgtcgc tgtcaactgt cactgtaata cgctgcttca
     8581 tagcatacct ctttttgaca tacttcgggt atacatatca gtatatattc ttataccgca
     8641 aaaatcagcg cgcaaatacg catactgtta tctggctttt agtaagccgg atccggtctc
     8701 tagccactgg gatcctcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
     8761 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
     8821 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
     8881 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag
     8941 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata
     9001 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat
     9061 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata
     9121 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt
     9181 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc
     9241 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg
     9301 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac
     9361 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat
     9421 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg
     9481 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg
     9541 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg
     9601 tatctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg
     9661 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg
     9721 gccagcaaaa ctaacccggg tgccggtaga ggtgtggtca ataagagcga cctcatacta
     9781 tacctgagaa agcaacctga cctacaggaa agagttactc aagaataaga attttcgttt
     9841 taaaacctaa gagtcacttt aaaatttgta tacacttatt ttttttataa cttatttaat
     9901 aataaaaatc ataaatcata agaaattcgc ttatttagaa gtggcgcgcc ggtccgttac
     9961 ttgtacagct cgtccatgcc gccggtggag tggcggccct cggcgcgttc gtactgttcc
    10021 acgatggtgt agtcctcgtt gtgggaggtg atgtccaact tgatgttgac gttgtaggcg
    10081 ccgggcagct gcacgggctt cttggccttg taggtggtct tgacctcagc gtcgtagtgg
    10141 ccgccgtcct tcagcttcag cctctgcttg atctcgccct tcagggcgcc gtcctcgggg
    10201 tacatccgct cggaggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg
    10261 gaggggaagt tggtgccgcg cagcttcacc ttgtagatga actcgccgtc ctgcagggag
    10321 gagtcctggg tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg
    10381 aagccctcgg ggaaggacag cttcaagtag tcggggatgt cggcggggtg cttcacgtag
    10441 gccttggagc cgtacatgaa ctgaggggac aggatgtccc aggcgaaggg cagggggcca
    10501 cccttggtca ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg
    10561 ccctcgatct cgaactcgtg gccgttcacg gagccctcca tgtgcacctt gaagcgcatg
    10621 aactccttga tgatggccat gttatcctcc tcgcccttgc tcaccatggt actagtgttt
    10681 agttaattat agttcgttga ccgtatattc taaaaacaag tactccttaa aaaaaaacct
    10741 tgaagggaat aaacaagtag aatagataga gagaaaaata gaaaatgcaa gagaatttat
    10801 atattagaaa gagagaaaga aaaatggaaa aaaaaaaata ggaaaagcca gaaatagcac
    10861 tagaaggagc gacaccagaa aagaaggtga tggaaccaat ttagctatat atagttaact
    10921 accggctcga tcatctctgc ctccagcata gtcgaagaag aatttttttt ttcttgaggc
    10981 ttctgtcagc aactcgtatt ttttctttct tttttggtga gcctaaaaag ttcccacgtt
    11041 ctcttgtacg acgccgtcac aaacaacctt atgggtaatt tgtcgcggtc tgggtgtata
    11101 aatgtgtggg tgcaacatga atgtacggag gtagtttgct gattggcggt ctatagatac
    11161 cttggttatg gcgccctcac agccggcagg ggaagcgcct acgcttgaca tctactatat
    11221 gtaagtatag acgt
//
