LOCUS       sequence-110562-        9212 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CEN plasmid as receiving vector for exogenous pathway.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 9212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: pRS414-URR
FEATURES             Location/Qualifiers
     source          1..9212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(83..671)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(163..182)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(842..1633,1634..1702))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1465..1484
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1703..1807)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    1844..2347
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     2389..2407
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2445..2467)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2567..2586
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2603..2883
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             2884..3558
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(3657..4112)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3794..3815)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3895..4473)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFA
                     GFPRQALNRGLPLGFRFSALRHLDPKKLD"
     primer_bind     4006..4025
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4242..4264
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4256..4273
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4257..4273
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4283..4302
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4283..4301
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    4310..4417
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     4326..4342
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4327..4343
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4377..4393)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4377..4393)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4430..4450)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4430..4448)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4469..4485)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4469..4485)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    4493..4509
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4517..4523,4524..4541,4542..4547))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(4700..4717)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(5792..6886)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(6899..7303)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     misc_feature    7592..7612
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      complement(7619..7646)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(7662..7733)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(7778..8455)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             8462..8473
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    8491..8507
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8515..8521,8522..8539,8540..8545))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8560..8581
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    8682..8703
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tgacggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
       61 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      121 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      181 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      241 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      301 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      361 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      421 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      481 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
      541 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
      601 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
      661 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
      721 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
      781 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
      841 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
      901 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
      961 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     1021 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1081 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1141 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1201 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1261 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1321 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     1381 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     1441 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     1501 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     1561 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     1621 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     1681 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     1741 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     1801 ttccgcgcac atttccccga aaagtgccac ctgggtcctt ttcatcacgt gctataaaaa
     1861 taattataat ttaaattttt taatataaat atataaatta aaaatagaaa gtaaaaaaag
     1921 aaattaaaga aaaaatagtt tttgttttcc gaagatgtaa aagactctag ggggatcgcc
     1981 aacaaatact accttttatc ttgctcttcc tgctctcagg tattaatgcc gaattgtttc
     2041 atcttgtctg tgtagaagac cacacacgaa aatcctgtga ttttacattt tacttatcgt
     2101 taatcgaatg tatatctatt taatctgctt ttcttgtcta ataaatatat atgtaaagta
     2161 cgctttttgt tgaaattttt taaacctttg tttatttttt tttcttcatt ccgtaactct
     2221 tctaccttct ttatttactt tctaaaatcc aaatacaaaa cataaaaata aataaacaca
     2281 gagtaaattc ccaaattatt ccatcattaa aagatacgag gcgcgtgtaa gttacaggca
     2341 agcgatccgt cctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     2401 acgaggccct ttagtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     2461 ctcccggaga ctgtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     2521 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     2581 attgtactga gagtgcacca taaacgacat tactatatat ataatatagg aagcatttaa
     2641 tagacagcat cgtaatatat gtgtactttg cagttatgac gccagatggc agtagtggaa
     2701 gatattcttt attgaaaaat agcttgtcac cttacgtaca atcttgatcc ggagcttttc
     2761 tttttttgcc gattaagaat taattcggtc gaaaaaagaa aaggagaggg ccaagaggga
     2821 gggcattggt gactattgag cacgtgagta tacgtgatta agcacacaaa ggcagcttgg
     2881 agtatgtctg ttattaattt cacaggtagt tctggtccat tggtgaaagt ttgcggcttg
     2941 cagagcacag aggccgcaga atgtgctcta gattccgatg ctgacttgct gggtattata
     3001 tgtgtgccca atagaaagag aacaattgac ccggttattg caaggaaaat ttcaagtctt
     3061 gtaaaagcat ataaaaatag ttcaggcact ccgaaatact tggttggcgt gtttcgtaat
     3121 caacctaagg aggatgtttt ggctctggtc aatgattacg gcattgatat cgtccaactg
     3181 catggagatg agtcgtggca agaataccaa gagttcctcg gtttgccagt tattaaaaga
     3241 ctcgtatttc caaaagactg caacatacta ctcagtgcag cttcacagaa acctcattcg
     3301 tttattccct tgtttgattc agaagcaggt gggacaggtg aacttttgga ttggaactcg
     3361 atttctgact gggttggaag gcaagagagc cccgaaagct tacattttat gttagctggt
     3421 ggactgacgc cagaaaatgt tggtgatgcg cttagattaa atggcgttat tggtgttgat
     3481 gtaagcggag gtgtggagac aaatggtgta aaagactcta acaaaatagc aaatttcgtc
     3541 aaaaatgcta agaaataggt tattactgag tagtatttat ttaagtattg tttgtgcact
     3601 tgcctatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
     3661 tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     3721 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     3781 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3841 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     3901 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3961 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     4021 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     4081 cgccgcgctt aatgcgccgc tacagggcgc gtcgcgccat tcgccattca ggctgcgcaa
     4141 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     4201 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     4261 aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg gtaccgggcc
     4321 ccccctcgag gtcgacggta tcgataagct tgatatcgaa ttcctgcagc ccgggggatc
     4381 cactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag
     4441 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     4501 cgctcacaat tccacacaac ataggagccg gaagcataaa gtgtaaagcc tggggtgcct
     4561 aatgagtgag gtaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     4621 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     4681 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     4741 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca gccgattagc
     4801 gtggcgagcc cgcctggttt tgggaactcg tgtttttaat tcgtttccga tcagcgcact
     4861 atactgaatt ttaaagccag acatgccgat attaaaccac tcaatttgac ctagtcctca
     4921 aacgttctgc taaaccgtgt caatcagtgt ctgcttcctg agtgaaacct ttccgccaaa
     4981 gtcgatggtt agaacctagg gtcgctttcg tccttggcta caccggtttc cttatgcagg
     5041 cagtaacgta gatccattcc gtagagcggc ttttcgcttg gacatgaaac atttccttga
     5101 tctccaggag aaatacgaga gagcaggcac gccgagtaaa tacgtatgct gctcatttgt
     5161 ccacgcaccc gcatacccct ggaaggaaag acacatgcta gtcgtcctga cgctagtgta
     5221 aaatacccaa gggaccccga cagtgcgatt tagcgagcct ctaactcttt cgttagctga
     5281 tgttactagc gagattgtac tgagagtgca ccatatcgac tacgtcgtaa ggccgtttct
     5341 gacagagtaa aattcttgag ggaactttca ccattatggg aaatggttca agaaggtatt
     5401 gacttaaact ccatcaaatg gtcaggtcat tgagtgtttt ttatttgttg tatttttttt
     5461 tttttagaga aaatcctcca atatcaaatt aggaatcgta gtttcatgat tttctgttac
     5521 acctaacttt ttgtgtggtg ccctcctcct tgtcaatatt aatgttaaag tgcaattctt
     5581 tttccttatc acgttgagcc attagtatca atttgcttac ctgtattcct ttactatcct
     5641 cctttttctc cttcttgata aatgtatgta gattgcgtat atagtttcgt ctaccctatg
     5701 aacatattcc attttgtaat ttcgtgtcgt ttctattatg aatttcattt ataaagttta
     5761 tgtacaaata tcataaaaaa agagaatctt tttaagcaag gattttctta acttcttcgg
     5821 cgacagcatc accgacttcg gtggtactgt tggaaccacc taaatcacca gttctgatac
     5881 ctgcatccaa aaccttttta actgcatctt caatggcctt accttcttca ggcaagttca
     5941 atgacaattt caacatcatt gcagcagaca agatagtggc gatagggtca accttattct
     6001 ttggcaaatc tggagcagaa ccgtggcatg gttcgtacaa accaaatgcg gtgttcttgt
     6061 ctggcaaaga ggccaaggac gcagatggca acaaacccaa ggaacctggg ataacggagg
     6121 cttcatcgga gatgatatca ccaaacatgt tgctggtgat tataatacca tttaggtggg
     6181 ttgggttctt aactaggatc atggcggcag aatcaatcaa ttgatgttga accttcaatg
     6241 tagggaattc gttcttgatg gtttcctcca cagtttttct ccataatctt gaagaggcca
     6301 aaacattagc tttatccaag gaccaaatag gcaatggtgg ctcatgttgt agggccatga
     6361 aagcggccat tcttgtgatt ctttgcactt ctggaacggt gtattgttca ctatcccaag
     6421 cgacaccatc accatcgtct tcctttctct taccaaagta aatacctccc actaattctc
     6481 tgacaacaac gaagtcagta cctttagcaa attgtggctt gattggagat aagtctaaaa
     6541 gagagtcgga tgcaaagtta catggtctta agttggcgta caattgaagt tctttacgga
     6601 tttttagtaa accttgttca ggtctaacac taccggtacc ccatttagga ccacccacag
     6661 cacctaacaa aacggcatca accttcttgg aggcttccag cgcctcatct ggaagtggga
     6721 cacctgtagc atcgatagca gcaccaccaa ttaaatgatt ttcgaaatcg aacttgacat
     6781 tggaacgaac atcagaaata gctttaagaa ccttaatggc ttcggctgtg atttcttgac
     6841 caacgtggtc acctggcaaa acgacgatct tcttaggggc agacataggg gcagacatta
     6901 gaatggtata tccttgaaat atatatatat attgctgaaa tgtaaaaggt aagaaaagtt
     6961 agaaagtaag acgattgcta accacctatt ggaaaaaaca ataggtcctt aaataatatt
     7021 gtcaacttca agtattgtga tgcaagcatt tagtcatgaa cgcttctcta ttctatatga
     7081 aaagccggtt ccggcctctc acctttcctt tttctcccaa tttttcagtt gaaaaaggta
     7141 tatgcgtcag gcgacctctg aaattaacaa aaaatttcca gtcatcgaat ttgattctgt
     7201 gcgatagcgc ccctgtgtgt tctcgttatg ttgaggaaaa aaataatggt tgctaagaga
     7261 ttcgaactct tgcatcttac gatacctgag tattcccaca gttaactgcg gtcaagatat
     7321 ttcttgaatc aggcgcctta gaccgctcgg ccaaacaacc aattacttgt tgagaaatag
     7381 agtataatta tcctataaat ataacgtttt tgaacacaca tgaacaagga agtacaggac
     7441 aattgatttt gaagagaatg tggattttga tgtaattgtt gggattccat ttttaataag
     7501 gcaataatat taggtatgtg gatatactag aagttctcct cgaccgtcga tatgcggtgt
     7561 gaaataccgc acagctcatg agaccgtctg actgcagcgg ccgctactag tatataaacg
     7621 cagaaaggcc cacccgaagg tgagccagtg tgactctagt agagagcgtt caccgacaaa
     7681 caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg
     7741 gctctagtag cgatctacac tagcactatc agcgttatta agcaccggtg gagtgacgac
     7801 cttcagcacg ttcgtactgt tcaacgatgg tgtagtcttc gttgtgggag gtgatgtcca
     7861 gtttgatgtc ggttttgtaa gcacccggca gctgaaccgg ttttttagcc atgtaggtgg
     7921 ttttaacttc agcgtcgtag tgaccaccgt ctttcagttt cagacgcatt ttgatttcac
     7981 ctttcagagc accgtcttcc gggtacatac gttcggtgga agcttcccaa cccatggttt
     8041 ttttctgcat aaccggaccg tcggacggga agttggtacc acgcagttta actttgtaga
     8101 tgaactcacc gtcttgcagg gaggagtcct gggtaacggt aacaacacca ccgtcttcga
     8161 agttcataac acgttcccat ttgaaacctt ccgggaagga cagtttcagg tagtccggga
     8221 tgtcagccgg gtgtttaacg taagctttgg aaccgtactg gaactgcggg gacaggatgt
     8281 cccaagcgaa cggcagcgga ccacctttgg taactttcag tttagcggtc tgggtacctt
     8341 cgtacggacg accttcacct tcaccttcga tttcgaactc gtgaccgtta acggaacctt
     8401 ccatacgaac tttgaaacgc atgaactctt tgataacgtc ttcggaggaa gccatctagt
     8461 atttctcctc tttctctagt atgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8521 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8581 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8641 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gctctagaag cggccgcgaa
     8701 ttccaatggg gtctcgaggt cctcgaagtt gcaacgccgt ccccacatcg ttgggtggtg
     8761 accggcaggt gtacaatgat acgaggtatt gaagattgaa ctaagtatac tttgcgcccg
     8821 ttctacatga aacaaactca gtcgaacgat gcaagaaccc gcattcggac caagttatga
     8881 gcgcgaactc ccgtttaaag cttttgtcgc tcagaaacct gagggtttag caacgtttca
     8941 cgtgggacat gcgaaccccc tgccaacgcc ggagtatggg aatgaacaaa tctgtcttcg
     9001 tgctaagggt gcggttagga cgctgaagta acgcaactta tatgcaaggc aaattatagc
     9061 agggactaaa ttccagtaag gtgggagatc gtgacccaaa ggtgactaac aaatattgtt
     9121 gtacatcccc ccaatcggtt ctgcggtcta cgagcaccag aatccggggc ctctaaagta
     9181 gagctaggtt cggacgcgcg actgtgctta ga
//
