LOCUS       sequence-110568-        3159 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  standard plasmids for exogenus pathway assembly haboring yeast 
            auxotrophic marker TRP1 which could be released and ligated to 
            series of TUs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: pMV-TRP1
FEATURES             Location/Qualifiers
     source          1..3159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..112
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     121..138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     122..138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(246..920)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(921..1201)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(1262..1278)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1262..1278)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1290..1307)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1461..2049)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1541..1560)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2220..3011,3012..3080))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2843..2862
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(3081..3159,1..26))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
       61 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
      121 tgtaaaacga cggccagtcg tctcgatccg cagtgtcttg cgtctcttga gctgtgcggt
      181 atttcacacc gcatagatcg gcaagtgcac aaacaatact taaataaata ctactcagta
      241 ataacctatt tcttagcatt tttgacgaaa tttgctattt tgttagagtc ttttacacca
      301 tttgtctcca cacctccgct tacatcaaca ccaataacgc catttaatct aagcgcatca
      361 ccaacatttt ctggcgtcag tccaccagct aacataaaat gtaagctttc ggggctctct
      421 tgccttccaa cccagtcaga aatcgagttc caatccaaaa gttcacctgt cccacctgct
      481 tctgaatcaa acaagggaat aaacgaatga ggtttctgtg aagctgcact gagtagtatg
      541 ttgcagtctt ttggaaatac gagtctttta ataactggca aaccgaggaa ctcttggtat
      601 tcttgccacg actcatctcc atgcagttgg acgatatcaa tgccgtaatc attgaccaga
      661 gccaaaacat cctccttagg ttgattacga aacacgccaa ccaagtattt cggagtgcct
      721 gaactatttt tatatgcttt tacaagactt gaaattttcc ttgcaataac cgggtcaatt
      781 gttctctttc tattgggcac acatataata cccagcaagt cagcatcgga atctagagca
      841 cattctgcgg cctctgtgct ctgcaagccg caaactttca ccaatggacc agaactacct
      901 gtgaaattaa taacagacat actccaagct gcctttgtgt gcttaatcac gtatactcac
      961 gtgctcaata gtcaccaatg ccctccctct tggccctctc cttttctttt ttcgaccgaa
     1021 ttaattctta atcggcaaaa aaagaaaagc tccggatcaa gattgtacgt aaggtgacaa
     1081 gctatttttc aataaagaat atcttccact actgccatct ggcgtcataa ctgcaaagta
     1141 cacatatatt acgatgctgt tctattaaat gcttcctata ttatatatat agtaatgtcg
     1201 tgatctatgg tgcactctca gtacaatctc gctagagacg gagtcactgc caaccgagac
     1261 ggtcatagct gtttcctgtg tgccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1321 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttacc cacagaatca
     1381 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1441 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1501 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1561 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1621 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     1681 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1741 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1801 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1861 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     1921 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1981 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2041 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     2101 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     2161 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     2221 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     2281 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     2341 agtgctgcaa taataccgcg ggacccacgc tcaccggctc cagatttatc agcaataaac
     2401 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     2461 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     2521 gttgttgcca tcgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     2581 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     2641 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgccgt gttatcactc
     2701 atggttatgg cagcactaca taattctctt actgtcatgc catccgtaag atgcttttct
     2761 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     2821 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     2881 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     2941 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     3001 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     3061 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     3121 tattgtctca tgagcggata catatttgaa tgtatttag
//
