LOCUS       sequence-110569-        2413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  construction of TDH3 promoter in HCKan-P receiving vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HCKan-P-TDH3
FEATURES             Location/Qualifiers
     source          1..2413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..671
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     541..564
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     terminator      721..752
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        753..855
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             856..1671
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(929..948)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1695..1722
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1734..2321)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1814..1833)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2343..2372)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 ggcttcatta tcaatactgc catttcaaag aatacgtaaa taattaatag tagtgatttt
       61 cctaacttta tttagtcaaa aaattagcct tttaattctg ctgtaacccg tacatgccca
      121 aaataggggg cgggttacac agaatatata acatcgtagg tgtctgggtg aacagtttat
      181 tcctggcatc cactaaatat aatggagccc gctttttaag ctggcatcca gaaaaaaaaa
      241 gaatcccagc accaaaatat tgttttcttc accaaccatc agttcatagg tccattctct
      301 tagcgcaact acagagaaca ggggcacaaa caggcaaaaa acgggcacaa cctcaatgga
      361 gtgatgcaac ctgcctggag taaatgatga cacaaggcaa ttgacccacg catgtatcta
      421 tctcattttc ttacaccttc tattaccttc tgctctctct gatttggaaa aagctgaaaa
      481 aaaaggttga aaccagttcc ctgaaattat tcccctactt gactaataag tatataaaga
      541 cggtaggtat tgattgtaat tctgtaaatc tatttcttaa acttcttaaa ttctactttt
      601 atagttagtc ttttttttag ttttaaaaca ccaagaactt agtttcgaat aaacacacat
      661 aaacaaacaa agatgcgaga cgactgacca tttaaatcat acctgacctc catagcagaa
      721 agtcaaaagc ctccgaccgg aggcttttga cttgatcggc acgtaagagg ttccaacttt
      781 caccataatg aaataagatc actaccgggc gtattttttg agttatcgag attttcagga
      841 gctaaggaag ctaaaatgag ccatattcaa cgggaaacgt cttgctcgag gccgcgatta
      901 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
      961 tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa
     1021 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcaggct aaactggctg
     1081 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     1141 ttactcacca ctgcgatccc agggaaaaca gcattccagg tattagaaga atatcctgat
     1201 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     1261 gtttgtaatt gtccttttaa cggcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     1321 atgaataacg gtttggttgg tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     1381 gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact
     1441 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     1501 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     1561 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     1621 cctgatatga ataaattgca gtttcacttg atgctcgatg agtttttcta atgagggccc
     1681 aaatgtaatc acctggctca ccttcgggtg ggcctttctg cgttgctggc gtttttccat
     1741 aggctccgcc cccctgacga gcatcacaaa aatcgatgct caagtcagag gtggcgaaac
     1801 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1861 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1921 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1981 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2041 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     2101 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     2161 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt tacctcggaa
     2221 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2281 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgattttct
     2341 accgaagaaa ggcccacccg tgaaggtgag ccagtgagtt gattgcagtc cagttacgct
     2401 ggagtccgtc tcg
//
