LOCUS       sequence-110572-        1933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  construction of ADH1 terminator in HCKan-P receiving vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HCKan-T-ADH1
FEATURES             Location/Qualifiers
     source          1..1933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5..191
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(59..83)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      241..272
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        273..375
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             376..1191
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(449..468)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1215..1242
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1254..1841)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1334..1353)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1863..1892)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 tagccgaatt tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt
       61 gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac
      121 tctttcctgt aggtcaggtt gctttctcag gtatagcatg aggtcgctct tattgaccac
      181 acctctaccg gcctccgaga cgactgacca tttaaatcat acctgacctc catagcagaa
      241 agtcaaaagc ctccgaccgg aggcttttga cttgatcggc acgtaagagg ttccaacttt
      301 caccataatg aaataagatc actaccgggc gtattttttg agttatcgag attttcagga
      361 gctaaggaag ctaaaatgag ccatattcaa cgggaaacgt cttgctcgag gccgcgatta
      421 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
      481 tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa
      541 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcaggct aaactggctg
      601 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
      661 ttactcacca ctgcgatccc agggaaaaca gcattccagg tattagaaga atatcctgat
      721 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
      781 gtttgtaatt gtccttttaa cggcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
      841 atgaataacg gtttggttgg tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
      901 gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact
      961 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     1021 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     1081 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     1141 cctgatatga ataaattgca gtttcacttg atgctcgatg agtttttcta atgagggccc
     1201 aaatgtaatc acctggctca ccttcgggtg ggcctttctg cgttgctggc gtttttccat
     1261 aggctccgcc cccctgacga gcatcacaaa aatcgatgct caagtcagag gtggcgaaac
     1321 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1381 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1441 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1501 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1561 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1621 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1681 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt tacctcggaa
     1741 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1801 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgattttct
     1861 accgaagaaa ggcccacccg tgaaggtgag ccagtgagtt gattgcagtc cagttacgct
     1921 ggagtccgtc tcg
//
