LOCUS       sequence-110573-        2115 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  construction of TEF2 terminator in HCKan-P receiving vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HCKan-T-TEF2
FEATURES             Location/Qualifiers
     source          1..2115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      423..454
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        455..557
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             558..1373
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(631..650)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1397..1424
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1436..2023)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1516..1535)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2045..2074)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 tagcagtaat aattattgct tccatataat atttttatat acctcttatt tttatgtatt
       61 agttaattaa gtatttttat ctatctgctt atcattttct tttcatatag ggggggttgg
      121 tgttttcttg cccatcagat tgatgtcctc caactcggca ctattttaca aagggttttt
      181 ttgtaagaga aggagaagac agatactaaa ccatacgtta ctcgaaacaa aaaaaaaaaa
      241 aatggaaaaa gctgctatca acaaaagacg gcctcatcaa acctaaagaa accatgtcag
      301 cgtatgtata taccttgtaa tttacgtttc cttaaatctt ctttctacta acgttttcat
      361 tattctatac tctcctccga gacgactgac catttaaatc atacctgacc tccatagcag
      421 aaagtcaaaa gcctccgacc ggaggctttt gacttgatcg gcacgtaaga ggttccaact
      481 ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag
      541 gagctaagga agctaaaatg agccatattc aacgggaaac gtcttgctcg aggccgcgat
      601 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
      661 aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga
      721 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcagg ctaaactggc
      781 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
      841 ggttactcac cactgcgatc ccagggaaaa cagcattcca ggtattagaa gaatatcctg
      901 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
      961 ctgtttgtaa ttgtcctttt aacggcgatc gcgtatttcg tctcgctcag gcgcaatcac
     1021 gaatgaataa cggtttggtt ggtgcgagtg attttgatga cgagcgtaat ggctggcctg
     1081 ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca
     1141 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
     1201 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
     1261 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
     1321 atcctgatat gaataaattg cagtttcact tgatgctcga tgagtttttc taatgagggc
     1381 ccaaatgtaa tcacctggct caccttcggg tgggcctttc tgcgttgctg gcgtttttcc
     1441 ataggctccg cccccctgac gagcatcaca aaaatcgatg ctcaagtcag aggtggcgaa
     1501 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1561 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1621 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1681 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1741 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1801 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1861 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttacctcgg
     1921 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1981 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatttt
     2041 ctaccgaaga aaggcccacc cgtgaaggtg agccagtgag ttgattgcag tccagttacg
     2101 ctggagtccg tctcg
//
