LOCUS       sequence-110575-        3842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  systhesis of crtI gene in the beta-carotene pathway.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: PMV-ycrtI
FEATURES             Location/Qualifiers
     source          1..3842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..112
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     121..138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     122..138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1945..1961)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1945..1961)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1973..1990)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2144..2732)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2224..2243)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2903..3694,3695..3763))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3526..3545
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(3764..3842,1..26))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
       61 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
      121 tgtaaaacga cggccagtcg tctcgatccg cagtgtcttg cgtctctatg ggtaaggaac
      181 aagaccaaga caagccaacc gctatcatcg ttggttgtgg tatcggtggt atcgctaccg
      241 ctgctagatt ggctaaggaa ggtttccaag ttaccgtttt cgaaaagaac gactactctg
      301 gtggtagatg ttctttgatc gaacgcgacg gttacagatt cgaccaaggt ccatctttgt
      361 tgttgttgcc agacttgttc aagcaaacct tcgaagactt gggtgaaaag atggaagact
      421 gggttgactt gatcaagtgt gaaccaaact acgtttgtca cttccacgac gaagaaacct
      481 tcaccttctc taccgacatg gctttgttga agagagaagt tgaaagattc gaaggtaagg
      541 acggtttcga cagattcttg tctttcatcc aagaagctca cagacactac gaattggctg
      601 ttgttcacgt tttgcaaaag aacttcccag gtttcgctgc tttcttgaga ttgcaattca
      661 tcggtcaaat cttggctttg cacccattcg aatctatctg gaccagagtt tgtagatact
      721 tcaagaccga cagattgaga agagttttct ctttcgctgt tatgtacatg ggtcaatctc
      781 catactctgc tccaggaacc tactctttgt tgcaatacac cgaattgacc gaaggtatct
      841 ggtatccaag aggtggtttc tggcaagttc caaacacctt gttgcaaatc gttaagagaa
      901 acaacccatc tgctaagttc aacttcaacg ctccagtttc tcaagttttg ttgtctccag
      961 ctaaggacag agctaccggt gttagattgg aatctggtga agaacaccac gctgacgttg
     1021 ttatcgttaa cgctgacttg gtttacgctt ctgaacactt gatcccagac gacgctagaa
     1081 acaagatcgg tcaattgggt gaagttaagc gatcttggtg ggctgacttg gttggtggta
     1141 agaagttgaa gggttcttgt tcttctttgt ctttctactg gtctatggac agaatcgttg
     1201 acggtttggg tggtcacaac atcttcttgg ctgaagactt caagggttct ttcgacacca
     1261 tcttcgaaga attgggtttg ccagctgacc catctttcta cgttaacgtt ccaagtagaa
     1321 tcgacccatc tgctgctcca gaaggtaagg acgctatcgt tatcttggtt ccatgtggtc
     1381 acatcgacgc ttctaaccca caagactaca acaagttggt tgctagagct agaaagttcg
     1441 ttatccaaac cttgtctgct aagttgggtt tgccagactt cgaaaagatg atcgttgctg
     1501 aaaaggttca cgacgctcca tcttgggaaa aggaattcaa cttgaaggac ggttctatct
     1561 tgggtttggc tcacaacttc atgcaagttt tgggtttcag accatctacc agacacccaa
     1621 agtacgacaa gttgttcttc gttggtgctt ctacccaccc aggaaccggt gttccaatcg
     1681 ttttggctgg tgctaagttg accgctaacc aagttttgga atctttcgac cgatctccag
     1741 ctccagaccc aaacatgtct ttgtctgttc catacggtaa gccattgaag tctaacggaa
     1801 ccggtatcga ctctcaagtt caattgaagt tcatggactt ggaaagatgg gtttacttgt
     1861 tggttttgtt gatcggtgct gttatcgctc gatctgttgg tgttttggct ttctaaagag
     1921 acggagtcac tgccaaccga gacggtcata gctgtttcct gtgtgccgct tcctcgctca
     1981 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2041 taatacggtt acccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2101 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2161 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2221 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2281 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
     2341 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2401 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2461 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2521 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2581 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2641 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2701 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2761 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2821 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2881 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2941 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3001 gggagggctt accatctggc cccagtgctg caataatacc gcgggaccca cgctcaccgg
     3061 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3121 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3181 cgccagttaa tagtttgcgc aacgttgttg ccatcgctac aggcatcgtg gtgtcacgct
     3241 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3301 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3361 agttggccgc cgtgttatca ctcatggtta tggcagcact acataattct cttactgtca
     3421 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3481 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3541 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3601 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3661 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3721 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3781 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3841 ag
//
