LOCUS       sequence-110576-        4115 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  systhesis of crtYB gene in the beta-carotene pathway.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: PMV-ycrtYB
FEATURES             Location/Qualifiers
     source          1..4115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..112
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     121..138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     122..138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2218..2234)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2218..2234)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2246..2263)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2417..3005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2497..2516)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3176..3967,3968..4036))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3799..3818
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(4037..4115,1..26))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
       61 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
      121 tgtaaaacga cggccagtcg tctcgatccg cagtgtcttg cgtctctatg accgctttgg
      181 cttactacca aatccacttg atctacacct tgccaatctt gggtttgttg ggtttgttga
      241 cctctccaat cttgaccaag ttcgacatct acaaaatctc tatcttggtt ttcatcgctt
      301 tctctgctac caccccatgg gactcttgga tcatcagaaa cggtgcttgg acctacccat
      361 ctgctgaatc tggtcaaggt gttttcggaa ccttcttgga cgttccatac gaagaatacg
      421 ctttcttcgt tatccaaacc gttatcacag gtttggttta cgttttggct accagacact
      481 tgttgccatc tttggctttg ccaaagaccc gatcttctgc tttgtctttg gctttgaagg
      541 ctttgatccc attgccaatc atctacttgt tcaccgctca cccatctcca tctccagacc
      601 cattggttac cgaccactac ttctacatga gagctttgtc tttgttgatc accccaccaa
      661 ccatgttgtt ggctgctttg tctggtgaat acgctttcga ctggaagtct ggtagagcta
      721 agtctaccat cgctgctatc atgatcccaa ccgtttactt gatctgggtt gactacgttg
      781 ctgttggtca agactcttgg tctatcaacg acgaaaagat cgttggttgg agattgggtg
      841 gtgttttgcc aatcgaagaa gctatgttct tcttgttgac caacttgatg atcgttttgg
      901 gtttgtctgc ttgtgaccac acccaggctt tgtacttgtt gcacggtaga accatctacg
      961 gtaacaagaa gatgccatct tctttcccat tgatcacccc accagttttg tctttgttct
     1021 tctctagtag accatactct tctcaaccaa agagagactt ggaattggct gttaagttgt
     1081 tggaagaaaa gtctcgatct ttcttcgttg cttctgctgg tttcccatct gaagttagag
     1141 aaagattggt tggtttgtac gctttctgta gagttaccga cgacttgatc gactctccag
     1201 aagtttcttc taacccacac gctaccatcg acatggtttc tgacttcttg accttgttgt
     1261 tcggtccacc attgcaccca tctcaaccag acaaaatctt gtcttctcca ttgttgccac
     1321 catctcaccc aagtagacca acaggtatgt acccattgcc accaccacca tctttgtctc
     1381 cagctgaatt ggttcaattc ttgaccgaaa gagttccagt tcaataccac ttcgctttca
     1441 gattgttggc taagttgcaa ggtttgatcc caagataccc attggacgaa ttgttgagag
     1501 gttacaccac cgacttgatc ttcccattgt ctaccgaagc tgttcaagct agaaagaccc
     1561 caatcgaaac caccgctgac ttgttggact acggtttgtg tgttgctggt tctgttgctg
     1621 aattgttggt ttacgtttct tgggcttctg ctccatctca agttccagct accatcgaag
     1681 aaagagaagc tgttttggtt gctagtagag aaatgggaac cgctttgcaa ttggttaaca
     1741 tcgctagaga catcaagggt gacgctaccg aaggtagatt ctacttgcca ttgtctttct
     1801 tcggtttgcg cgacgaatct aagttggcta tcccaaccga ctggaccgaa ccaagaccac
     1861 aagacttcga caagttgttg tctttgtctc catcttctac cttgccatct tctaacgctt
     1921 ctgaatcttt cagattcgaa tggaagacct actctttgcc attggttgct tacgctgaag
     1981 acttggctaa gcactcttac aagggtatcg acagattgcc aaccgaagtt caagctggta
     2041 tgagagctgc ttgtgcttct tacttgttga tcggtagaga aatcaaggtt gtttggaagg
     2101 gtgacgttgg tgaaagaaga accgttgctg gttggagaag agttagaaag gttttgtctg
     2161 ttgttatgtc tggttgggaa ggtcaataaa gagacggagt cactgccaac cgagacggtc
     2221 atagctgttt cctgtgtgcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     2281 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttacccaca gaatcagggg
     2341 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2401 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2461 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2521 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2581 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     2641 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2701 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2761 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2821 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     2881 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2941 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3001 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3061 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3121 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3181 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3241 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3301 ctgcaataat accgcgggac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3361 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3421 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     3481 ttgccatcgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3541 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3601 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgccgtgtta tcactcatgg
     3661 ttatggcagc actacataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3721 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3781 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3841 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     3901 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     3961 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4021 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4081 gtctcatgag cggatacata tttgaatgta tttag
//
