LOCUS       sequence-110578-        3489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  construction of ycrtI gene in HCKan-O receiving vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HCKan-O-ycrtI
FEATURES             Location/Qualifiers
     source          1..3489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1797..1828
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1829..1931
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1932..2747
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2005..2024)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2771..2798
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2810..3397)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2890..2909)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3419..3448)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 gatgggtaag gaacaagacc aagacaagcc aaccgctatc atcgttggtt gtggtatcgg
       61 tggtatcgct accgctgcta gattggctaa ggaaggtttc caagttaccg ttttcgaaaa
      121 gaacgactac tctggtggta gatgttcttt gatcgaacgc gacggttaca gattcgacca
      181 aggtccatct ttgttgttgt tgccagactt gttcaagcaa accttcgaag acttgggtga
      241 aaagatggaa gactgggttg acttgatcaa gtgtgaacca aactacgttt gtcacttcca
      301 cgacgaagaa accttcacct tctctaccga catggctttg ttgaagagag aagttgaaag
      361 attcgaaggt aaggacggtt tcgacagatt cttgtctttc atccaagaag ctcacagaca
      421 ctacgaattg gctgttgttc acgttttgca aaagaacttc ccaggtttcg ctgctttctt
      481 gagattgcaa ttcatcggtc aaatcttggc tttgcaccca ttcgaatcta tctggaccag
      541 agtttgtaga tacttcaaga ccgacagatt gagaagagtt ttctctttcg ctgttatgta
      601 catgggtcaa tctccatact ctgctccagg aacctactct ttgttgcaat acaccgaatt
      661 gaccgaaggt atctggtatc caagaggtgg tttctggcaa gttccaaaca ccttgttgca
      721 aatcgttaag agaaacaacc catctgctaa gttcaacttc aacgctccag tttctcaagt
      781 tttgttgtct ccagctaagg acagagctac cggtgttaga ttggaatctg gtgaagaaca
      841 ccacgctgac gttgttatcg ttaacgctga cttggtttac gcttctgaac acttgatccc
      901 agacgacgct agaaacaaga tcggtcaatt gggtgaagtt aagcgatctt ggtgggctga
      961 cttggttggt ggtaagaagt tgaagggttc ttgttcttct ttgtctttct actggtctat
     1021 ggacagaatc gttgacggtt tgggtggtca caacatcttc ttggctgaag acttcaaggg
     1081 ttctttcgac accatcttcg aagaattggg tttgccagct gacccatctt tctacgttaa
     1141 cgttccaagt agaatcgacc catctgctgc tccagaaggt aaggacgcta tcgttatctt
     1201 ggttccatgt ggtcacatcg acgcttctaa cccacaagac tacaacaagt tggttgctag
     1261 agctagaaag ttcgttatcc aaaccttgtc tgctaagttg ggtttgccag acttcgaaaa
     1321 gatgatcgtt gctgaaaagg ttcacgacgc tccatcttgg gaaaaggaat tcaacttgaa
     1381 ggacggttct atcttgggtt tggctcacaa cttcatgcaa gttttgggtt tcagaccatc
     1441 taccagacac ccaaagtacg acaagttgtt cttcgttggt gcttctaccc acccaggaac
     1501 cggtgttcca atcgttttgg ctggtgctaa gttgaccgct aaccaagttt tggaatcttt
     1561 cgaccgatct ccagctccag acccaaacat gtctttgtct gttccatacg gtaagccatt
     1621 gaagtctaac ggaaccggta tcgactctca agttcaattg aagttcatgg acttggaaag
     1681 atgggtttac ttgttggttt tgttgatcgg tgctgttatc gctcgatctg ttggtgtttt
     1741 ggctttctag ccgagacgac tgaccattta aatcatacct gacctccata gcagaaagtc
     1801 aaaagcctcc gaccggaggc ttttgacttg atcggcacgt aagaggttcc aactttcacc
     1861 ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta
     1921 aggaagctaa aatgagccat attcaacggg aaacgtcttg ctcgaggccg cgattaaatt
     1981 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     2041 gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     2101 gcaaaggtag cgttgccaat gatgttacag atgagatggt caggctaaac tggctgacgg
     2161 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     2221 tcaccactgc gatcccaggg aaaacagcat tccaggtatt agaagaatat cctgattcag
     2281 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     2341 gtaattgtcc ttttaacggc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
     2401 ataacggttt ggttggtgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     2461 aagtctggaa agaaatgcat aagcttttgc cattctcacc ggattcagtc gtcactcatg
     2521 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     2581 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     2641 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     2701 atatgaataa attgcagttt cacttgatgc tcgatgagtt tttctaatga gggcccaaat
     2761 gtaatcacct ggctcacctt cgggtgggcc tttctgcgtt gctggcgttt ttccataggc
     2821 tccgcccccc tgacgagcat cacaaaaatc gatgctcaag tcagaggtgg cgaaacccga
     2881 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     2941 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3001 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3061 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3121 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3181 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3241 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc tcggaaaaag
     3301 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3361 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga ttttctaccg
     3421 aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag
     3481 tccgtctcg
//
