LOCUS       sequence-110580-        3316 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  systhesis of vioA gene in the violacein pathway.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: pKMV-VioA
FEATURES             Location/Qualifiers
     source          1..3316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     50..68
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     77..94
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     78..94
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1420..1436)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1420..1436)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1448..1465)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1619..2207)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1699..1718)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2378..3193)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2378..3193)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3101..3120
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(3194..3298)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
       61 cacgaggccc tttcgttgta aaacgacggc cagtcgtctc gatccgcagt gtcttgcgtc
      121 tctgatgaag cactcttctg acatctgtat cgttggtgct ggtatctctg gtttgacctg
      181 tgcttctcac ttgttggact ctccagcttg tagaggtttg tctttgagaa tcttcgacat
      241 gcaacaagaa gctggtggta gaatccgatc taagatgttg gacggtaagg cttctatcga
      301 attgggtgct ggtagatact ctccacaatt gcacccacac ttccaatctg ctatgcaaca
      361 ctactctcaa aagtctgaag tttacccatt cacccaattg aagttcaagt ctcacgttca
      421 acaaaagttg aagagagcta tgaacgaatt gtctccaaga ttgaaggaac acggtaagga
      481 atctttcttg caattcgtta gtagatacca aggtcacgac tctgctgttg gtatgatccg
      541 atctatgggt tacgacgctt tgttcttgcc agacatctct gctgaaatgg cttacgacat
      601 cgttggtaag cacccagaaa tccaatctgt taccgacaac gacgctaacc aatggttcgc
      661 tgctgaaaca ggtttcgctg gtttgatcca aggtatcaag gctaaggtta aggctgctgg
      721 tgctagattc tctttgggtt acagattgtt gtctgttaga accgacggtg acggttactt
      781 gttgcaattg gctggtgacg acggttggaa gttggaacac agaaccagac acttgatctt
      841 ggctatccca ccatctgcta tggctggttt gaacgttgac ttcccagagg cttggtctgg
      901 tgctagatac ggttctttgc cattgttcaa gggtttcttg acctacggtg aaccatggtg
      961 gttggactac aagttggacg accaagtttt gatcgttgac aacccattga gaaaaatcta
     1021 cttcaagggt gacaagtact tgttcttcta caccgactct gaaatggcta actactggag
     1081 aggttgtgtt gctgaaggtg aagacggtta cttggaacaa atcagaaccc acttggcttc
     1141 tgctttgggt atcgttagag aaagaatccc acaaccattg gctcacgttc acaagtactg
     1201 ggctcacggt gttgagttct gtagagactc tgacatcgac cacccatctg ctttgtctca
     1261 cagagactct ggtatcatcg cttgttctga cgcttacacc gaacactgtg gttggatgga
     1321 aggtggtttg ttgtctgcta gagaggctag tagattgttg ttgcaaagaa tcgctgcttt
     1381 gagaagatag cagagacgga gtcactgcca accgagacgg tcatagctgt ttcctgtgtg
     1441 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1501 ctcactcaaa ggcggtaata cggttaccca cagaatcagg ggataacgca ggaaagaaca
     1561 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1621 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1681 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1741 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1801 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1861 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1921 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1981 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2041 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2101 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2161 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2221 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2281 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2341 caatctaaag tatatatgag taaacttggt ctgacagtta gaaaaactca tcgagcatca
     2401 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt
     2461 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
     2521 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
     2581 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa
     2641 gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
     2701 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgagt cgaaatacgc
     2761 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacacgg
     2821 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg
     2881 ttttcccggg gatcgctgtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct
     2941 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa
     3001 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
     3061 catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc
     3121 catataaatc agcatccatg ttggaattta atcgcggcct agagcaagac gtttcccgtt
     3181 gaatatggct catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3241 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3301 catttccccg aaaagt
//
