LOCUS       sequence-110581-        5050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  systhesis of vioB gene in the violacein pathway.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: pKMV-VioB
FEATURES             Location/Qualifiers
     source          1..5050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     50..68
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     77..94
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     78..94
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3154..3170)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3154..3170)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3182..3199)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3353..3941)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3433..3452)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4112..4927)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4112..4927)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4835..4854
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(4928..5032)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
       61 cacgaggccc tttcgttgta aaacgacggc cagtcgtctc gatccgcagt gtcttgcgtc
      121 tctgatgagc attctggatt tcccgcgtat ccacttccgt ggctgggccc gtgtcaatgc
      181 gccgaccgcg aaccgcgatc cgcacggcca catcgatatg gccagcaata ccgtggcgat
      241 ggcgggtgag ccgttcgacc tggcacgcca tcctacggag ttccaccgtc acctgcgctc
      301 cctgggtccg cgcttcggct tggatggtcg tgctgacccg gaaggcccgt tcagcctggc
      361 cgagggctac aacgctgccg gtaacaacca cttttcgtgg gagagcgcaa ccgttagcca
      421 cgtgcaatgg gatggcggtg aggcggatcg tggtgacggt ctggtcggtg ctcgtttggc
      481 actgtggggt cactacaatg attatctgcg taccaccttc aatcgtgctc gttgggtcga
      541 cagcgacccg acgcgccgtg acgctgcaca aatctatgcg ggccaattca ccattagccc
      601 ggctggtgcc ggtccgggta cgccgtggct gtttacggca gacattgatg atagccatgg
      661 tgcacgttgg acgcgtggcg gccacattgc agagcgtggc ggccacttct tggatgaaga
      721 gtttggtctg gcacgcctgt ttcagttctc tgtgccgaaa gatcacccac attttctgtt
      781 tcacccgggt ccgtttgatt ccgaggcctg gcgtcgtctg caattggctc tggaggatga
      841 cgacgttctg ggtctgaccg tgcaatatgc gttgttcaat atgagcaccc cgcctcagcc
      901 gaacagcccg gtttttcacg atatggtcgg tgttgtcggt ctgtggcgtc gtggtgaact
      961 ggcgagctac ccggctggtc gtctgctgcg tccgcgtcaa ccgggtctgg gtgacctgac
     1021 cctgcgcgtc aacggtggtc gcgttgcgct gaatttggcg tgtgccattc cgttcagcac
     1081 tcgtgccgcg cagccaagcg caccggaccg cctgaccccg gacctgggtg ccaaactgcc
     1141 gctgggcgat ctgctgctgc gtgatgagga cggcgcactg ttggcacgtg tgccgcaggc
     1201 tctgtaccaa gactattgga cgaatcacgg tattgtggac ctgccgctgc tgcgcgaacc
     1261 gcgtggtagc ttgaccctga gcagcgaact ggcggagtgg cgtgagcaag actgggtcac
     1321 ccaaagcgac gcgtctaacc tgtacctgga ggcaccggat cgccgtcacg gtcgcttttt
     1381 ccctgagagc atcgcgctgc gcagctactt tcgcggtgaa gcgcgtgcgc gtccggatat
     1441 cccgcatcgt atcgagggca tgggcctggt cggcgtcgaa tctcgtcagg atggcgacgc
     1501 tgcggaatgg cgtctgacgg gtctgcgtcc gggtccggca cgcattgttc tggacgatgg
     1561 tgccgaggcg atccctctgc gtgttctgcc tgacgattgg gcgctggatg acgcgaccgt
     1621 cgaagaagtg gattacgcct ttttgtaccg ccacgttatg gcgtattacg agctggtgta
     1681 tccattcatg agcgacaagg tgttttccct ggctgatcgt tgcaaatgtg aaacgtacgc
     1741 acgtctgatg tggcagatgt gtgatccgca gaaccgcaac aagtcctatt acatgccgag
     1801 cacccgcgaa ctgtcggcac cgaaagctcg tttgttcttg aagtatctgg cccacgtgga
     1861 aggccaggca cgcctgcaag cacctccgcc agcgggtccg gcacgcattg aatctaaagc
     1921 ccagttggcg gcagagctgc gtaaagccgt cgacctggag ctgtctgtga tgctgcaata
     1981 cctgtacgcg gcgtatagca ttccgaacta tgcacagggc caacaacgtg ttcgtgacgg
     2041 tgcgtggacc gccgagcagc tgcaactggc gtgcggtagc ggtgaccgtc gccgtgatgg
     2101 cggtattcgt gcagcactgc tggaaattgc tcatgaagaa atgattcatt acctggtcgt
     2161 taacaacctg ctgatggccc tgggcgagcc gttctacgcg ggtgtcccgc tgatgggcga
     2221 agcggcacgt caggcgtttg gcctggacac cgagttcgct ctggaaccgt ttagcgaaag
     2281 cacgctggca cgttttgttc gtctggaatg gccgcacttt atcccagcac cgggcaaatc
     2341 catcgcggac tgctatgccg ccattcgtca ggcgtttttg gatctgccgg acttgtttgg
     2401 tggcgaggca ggtaagcgtg gcggtgaaca ccacctgttc ctgaatgagc tgaccaaccg
     2461 tgcgcatccg ggttatcaac tggaagtttt cgatcgcgac tcggcgctgt ttggtattgc
     2521 atttgtgacc gatcagggcg aaggtggcgc tctggacagc ccgcactacg aacatagcca
     2581 ttttcaacgt ctgcgtgaaa tgagcgcgcg tatcatggct caaagcgcac cgttcgaacc
     2641 ggcgctgccg gcgttgcgta atccggttct ggatgagagc ccgggttgcc aacgtgtcgc
     2701 agacggtcgt gcgcgtgcgc tgatggcatt gtaccaaggc gtttatgagc tgatgtttgc
     2761 gatgatggcg cagcacttcg ccgtgaaacc gctgggtagc ttgcgtcgca gccgcctgat
     2821 gaacgcagca atcgatctga tgaccggtct gttgcgtccg ctgagctgcg cgctgatgaa
     2881 cctgccaagc ggcatcgccg gtcgcacggc cggtccgccg ctgccgggtc cggttgacac
     2941 ccgtagctat gacgactacg cgctgggctg tcgcatgctg gcacgccgtt gcgagcgtct
     3001 gctggagcag gcgagcatgc tggaaccggg ttggctgccg gatgcgcaga tggagctgct
     3061 ggatttctat cgtcgccaaa tgctggactt ggcgtgcggc aaactgagcc gcgaggccta
     3121 atagcagaga cggagtcact gccaaccgag acggtcatag ctgtttcctg tgtgccgctt
     3181 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     3241 caaaggcggt aatacggtta cccacagaat caggggataa cgcaggaaag aacatgtgag
     3301 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     3361 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3421 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3481 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3541 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3601 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3661 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3721 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3781 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3841 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3901 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3961 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     4021 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     4081 aaagtatata tgagtaaact tggtctgaca gttagaaaaa ctcatcgagc atcaaatgaa
     4141 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     4201 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     4261 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     4321 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat
     4381 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     4441 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagtcgaaat acgcgatcgc
     4501 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac acggccagcg
     4561 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
     4621 cggggatcgc tgtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     4681 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     4741 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     4801 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     4861 aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc cgttgaatat
     4921 ggctcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4981 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     5041 cccgaaaagt
//
