LOCUS       sequence-110582-        3340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  systhesis of vioC gene in the violacein pathway.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: pKMV-VioC
FEATURES             Location/Qualifiers
     source          1..3340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     50..68
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     77..94
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     78..94
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1444..1460)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1444..1460)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1472..1489)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1643..2231)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1723..1742)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2402..3217)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2402..3217)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3125..3144
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(3218..3322)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
       61 cacgaggccc tttcgttgta aaacgacggc cagtcgtctc gatccgcagt gtcttgcgtc
      121 tctgatgaaa agagctatca tcgttggtgg tggtttggct ggtggtttga ccgctatcta
      181 cttggctaag agaggttacg aagttcacgt tgttgaaaag agaggtgacc cattgagaga
      241 cttgtcttct tacgttgacg ttgtttctag tagagctatc ggtgtttcta tgaccgttag
      301 aggtatcaag tctgttttgg ctgctggtat cccaagagct gaattggacg cttgtggtga
      361 accaatcgtt gctatggctt tctctgttgg tggtcaatac agaatgagag aattgaagcc
      421 attggaagac ttcagaccat tgtctttgaa cagagctgct ttccaaaagt tgttgaacaa
      481 gtacgctaac ttggctggtg ttagatacta cttcgaacac aagtgtttgg acgttgactt
      541 ggacggtaag tctgttttga tccaaggtaa ggacggtcaa ccacaaagat tgcaaggtga
      601 catgatcatc ggtgctgacg gtgctcactc tgctgttaga caagctatgc aatctggttt
      661 gagaagattc gagttccaac aaaccttctt cagacacggt tacaagacct tggttttgcc
      721 agacgctcag gctttgggtt acagaaagga caccttgtac ttcttcggta tggactctgg
      781 tggtttgttc gctggtagag ctgctaccat cccagacggt tctgtttcta tcgctgtttg
      841 tttgccatac tctggttctc catctttgac caccaccgac gaaccaacca tgagagcttt
      901 cttcgacaga tacttcggtg gtttgccacg cgacgctcgc gacgaaatgt tgagacaatt
      961 cttggctaag ccatctaacg acttgatcaa cgttcgatct tctaccttcc actacaaggg
     1021 taacgttttg ttgttgggtg acgctgctca cgctaccgct ccattcttgg gtcaaggtat
     1081 gaacatggct ttggaagacg ctagaacctt cgttgaattg ttggacagac accaaggtga
     1141 ccaagacaag gctttcccag agttcaccga attgagaaag gttcaagctg acgctatgca
     1201 agacatggct agagctaact acgacgtttt gtcttgttct aacccaatct tcttcatgag
     1261 agctagatac accagataca tgcactctaa gttcccaggt ttgtacccac cagacatggc
     1321 tgaaaagttg tacttcacct ctgaaccata cgacagattg caacaaatcc aaagaaagca
     1381 aaacgtttgg tacaagatcg gtagagttaa ctagcagaga cggagtcact gccaaccgag
     1441 acggtcatag ctgtttcctg tgtgccgctt cctcgctcac tgactcgctg cgctcggtcg
     1501 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta cccacagaat
     1561 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1621 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     1681 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1741 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     1801 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     1861 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1921 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1981 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2041 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     2101 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2161 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2221 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     2281 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     2341 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     2401 gttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
     2461 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
     2521 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
     2581 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
     2641 ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc
     2701 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
     2761 gcgcctgagc gagtcgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
     2821 aatgcaaccg gcgcaggaac acggccagcg catcaacaat attttcacct gaatcaggat
     2881 attcttctaa tacctggaat gctgttttcc cggggatcgc tgtggtgagt aaccatgcat
     2941 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
     3001 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
     3061 acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga
     3121 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
     3181 gcctagagca agacgtttcc cgttgaatat ggctcatact cttccttttt caatattatt
     3241 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3301 ataaacaaat aggggttccg cgcacatttc cccgaaaagt
//
