LOCUS       sequence-110599-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: myr-FLAG-PIK3CA-pcw107
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..84)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    92..116
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      175..184
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtaatccaga ggttgattgt cgacttaacg cggtcattac tacgtagaat cgagaccgag
       61 gagagggtta gggataggct taccaccggt aaccactttg tacaagaaag ttggcaatca
      121 gttcaaagca tgctgcttaa ttgtgtggaa gatccaatcc atttttgttg tccagccacc
      181 atggtgtgca tcattcattt gtttcatgaa atactccaaa gcctcttgct cagttttatc
      241 taaagctagg gtctttcgaa tgtatgcaat atcatcaaaa gattgcagtt ctggcattcc
      301 agagccaagc atcattgaga aaagatttat gaagagattg gcatgctgcc gaatagctag
      361 ataagccttg taacacatct cctgaaacct ctcaaattct cttgtctttg tgcattcttg
      421 ggctccttta ctaatcacta ttaagaaatc ttgtgtcaaa acaaacggca cgcgctctcg
      481 tttataacca aattttttct tcttgtgatc caaaaagtgt ccaaaatcta tatgaaacag
      541 ttgtccatca tctttaacca tgatattact attgtgacga tctccaattc ccaaaatgaa
      601 ggtggcaaca caatatccag cacatgatcg tgtaaacaaa tcgatggccg catcatatat
      661 ttcccccttg ttcttgtctt tgagccactg atggagtgtg tggctgttaa actgcagtgc
      721 acctttcagg cctcctttac actgaatctg cattatagtg tgagaatttc tcaccacctc
      781 gataagt
//
