LOCUS       sequence-110600-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knockdown of human FOXH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Takahashi K, Tanabe K, Ohnuki M, Narita M, Sasaki A, Yamamoto M, 
            Nakamura M, Sutou K, Osafune K, Yamanaka S
  TITLE     Induction of pluripotency in human somatic cells via a transient 
            state resembling primitive streak-like mesendoderm.
  JOURNAL   Nat Commun. 2014 Apr 24;5:3678. doi: 10.1038/ncomms4678.
  PUBMED    24759836
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-04-24"; volume: "5:3678. doi"; pages: " 
            10.1038/ncomms4678"
COMMENT     Sequence Label: pMKO.1-puro/hFOXH1 shRNA6
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        68..316
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     68..88
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     239..258
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(401..421)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        406..735
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      586..721
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(745..764)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tttatccagc cctcactcct tctctaggcg ccggaattga agatctgtcg acaaggtcgg
       61 gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt
      121 tagagagata attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
      181 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
      241 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
      301 gaaaggacga aacaccggtg cagcctgtga ggctcttaac tcgagttaag agcctcacag
      361 gctgcatttt tgaattcgcc agcacagtgg tcgaccctgt ggaatgtgtg tcagttaggg
      421 tgtggaaagt ccccaggctt cccagcaggc agaagtatgc aaagcatgca tctcaattag
      481 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
      541 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
      601 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
      661 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
      721 ctaggctttt gcaaaaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
      781 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
      841 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
      901 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
      961 gtggcggtct ggaccacgcc ggagagcgtc gaagcgggg
//
