LOCUS       sequence-110601-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Singlet donor (phase 1) for in vivo RMCE with MiMIC inserts to make 
            Gal4 driver..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pC-(loxP2-attB2-SA(1)-T2A-Gal4-Hsp70)
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     193..211
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    complement(243..276)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    392..461
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 aggattctgg gcgagaggag gcaacagcgc tgactttgag tggaatgtca ttttgagtga
       61 gaggtaatcg aaagaacctg gtacatcaaa tacccttgga tcgaagtaaa tttaaaactg
      121 atcagataag ttcaatgata tccagtgcag taaaaaaaaa aaatgttttt tttatctact
      181 ttccgcaaaa atgggtttta ttaacttaca tacatactag aattaggcct tctagtggat
      241 ctataacttc gtatagcata cattatacga agttattcaa atttaaataa taacaattat
      301 aatttttact ctggcataat tgtatgcaat ttgcaaatgc agcttcgttt tttaggggtg
      361 tgaatattta ttgtatgatg taggtcacgg tctcgaagcc gcggtgcggg tgccagggcg
      421 tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc atgatggtcg
      481 acaagcttag aagttcaaat gggctttcat atttactttt ttctctgagt gggtaaggtc
      541 attgtggggt cttaagggct aaagggtagt gatgatgaag gcatttgatg ccattgttca
      601 ataacttacc tccggaacct ccgcttccac cgctacctcc ggatccaaac gagtttttaa
      661 gcaaactcac tccctgacaa taaaaacgaa ttatctttaa ataaatatat tctatttatt
      721 aaccaagtag ctaatatagc taaaatcaat ttgttgctaa cttaaaacta acataattat
      781 gtattagtta tttacattct tttggttggt tacaaccaaa taaataatat cacttttaaa
      841 aacttaagcc agaactgaat aatttctatt tggcttttag
//
