LOCUS       sequence-110602-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid encodes mature form of murine VEGF-C preceded by honey bee 
            melittin secretion signal; entry vector to Gateway cloning system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Klaus T, Kulesza M, Bzowska M, Wyroba B, Kilarski WW, Bereta J
  TITLE     Overcoming inefficient secretion of recombinant VEGF-C in 
            baculovirus expression vector system by simple purification of the 
            protein from cell lysate.
  JOURNAL   Protein Expr Purif. 2015 Mar 7. pii: S1046-5928(15)00040-6. doi: 
            10.1016/j.pep.2015.03.001.
  PUBMED    25758709
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif. 2015 Mar 7. pii: S1046-5928(15)00040-6. doi: 
            10.1016/j.pep.2015.03.001."
COMMENT     Sequence Label: HBM-mVEGF-C-HisTag-pENTR
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(392..419)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(511..597)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     646..668
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     660..677
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     661..677
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    693..792
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     sig_peptide     813..875
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
                     /translation="MKFLVNVALVFMVVYISYIYA"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      241 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      301 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
      361 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
      421 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
      481 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
      541 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
      601 cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt
      661 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
      721 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      781 aaaaagcagg ctccgcggcc gcccccttca ccatgaaatt cttagtcaac gttgcccttg
      841 tttttatggt cgtatacatt tcttacatct atgccggatc cgcacattat aacacagaga
      901 tcctgaaaag tattgataat gagtggagaa agactcaatg catgccacgt gaggtgtgta
      961 tagatgtggg gaaggagttt ggagcagcca caa
//
