LOCUS       sequence-110603-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic
            loci using the Crispr/Cas technology. Can be converted by cassette 
            exchange into other Trojan exons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pT-GEM(2)
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    36..135
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     CDS             282..335
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             336..776
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 actatagggc gacccaccgg tgcatgcgcg gccgccccag gtcagaagcg gttttcggga
       61 gtagtgcccc aactggggta acctttgagt tctctcagtt gggggcgtag ggtcgccgac
      121 atgacacaag gggtttctag aagtcgatcc aacatggcga cttgtcccat ccccggcatg
      181 tttaaatata ctaattattc ttgaactaat tttaatcaac cgatttatct ctcttccgca
      241 ggtcgggagg ttccggtgga agcggaggta gcggcggatc cgagggccgc ggcagcctgc
      301 tgacctgcgg cgatgtggag gagaaccccg ggcccatgaa gctgctgagc agcatcgagc
      361 aggcctgcga tatctgccgc ctgaagaagc tgaagtgcag caaggagaag cccaagtgcg
      421 ccaagtgcct gaagaacaac tgggagtgcc gctacagccc caagaccaag cgcagccccc
      481 tgacccgcgc ccacctgacc gaggtggaga gccgcctgga gcgcctggag cagctgttcc
      541 tgctgatctt cccccgcgag gatctggata tgatcctgaa gatggatagc ctgcaggata
      601 tcaaggccct gctgaccggc ctgttcgtgc aggataacgt gaacaaggat gccgtgaccg
      661 atcgcctggc cagcgtggag accgatatgc ccctgaccct gcgccagcac cgcatcagcg
      721 ccaccagcag cagcgaggag agcagcaaca agggccagcg ccagctgacc gtgagcatcg
      781 atagcgccgc ccaccacgat aacagcacca tccccctgga tttcatgccc cgcgatgc
//
