LOCUS       sequence-110605-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Triplet donor for in vivo RMCE with MiMIC inserts to make Gal4 
            driver..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pC-(lox2-attB2-SA-T2A-Gal4-Hsp70)3
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..44
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    160..229
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             402..455
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ccccctcgag ataacttcgt ataaagtatc ctatacgaag ttatcaattg gcttattggg
       61 atttcaattt aagatgctgc taaataaagt tagtcactta cggactgggg aggcaccccg
      121 tcatcactcg ccagaaatgc aattgatgta ggtcacggtc tcgaagccgc ggtgcgggtg
      181 ccagggcgtg cccttgggct ccccgggcgc gtactccacc tcacccatct ggtccatcat
      241 gatggtcgac tctagaagtc gatccaacat ggcgacttgt cccatccccg gcatgtttaa
      301 atatactaat tattcttgaa ctaattttaa tcaaccgatt tatctctctt ccgcaggtgg
      361 gaggttccgg tggaagcgga ggtagcggca ccggtgcatg cgagggccgc ggcagcctgc
      421 tgacctgcgg cgatgtggag gagaaccccg ggcccatgaa gctgctgagc agcatcgagc
      481 aggcctgcga tatctgccgc ctgaagaagc tgaagtgcag caaggagaag cccaagtgcg
      541 ccaagtgcct gaagaacaac tgggagtgcc gctacagccc caagaccaag cgcagccccc
      601 tgacccgcgc ccacctgacc gaggtggaga gccgcctgga gcgcctggag cagctgttcc
      661 tgctgatctt cccccgcgag gatctggata tgatcctgaa gatggatagc ctgcaggata
      721 tcaaggccct gctgaccggc ctgttcgtgc aggataacgt gaacaaggat gccgtgaccg
      781 atcgcctggc cagcgtggag accgatatgc ccctgaccct gcgccagcac cgcatcagcg
      841 ccaccagcag cagcgaggag agcagcaaca agggccagcg ccagctg
//
