LOCUS       sequence-110606-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid encodes mature form of murine VEGF-C preceded by honey bee 
            melittin secretion signal; entry vector to Gateway cloning system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Klaus T, Kulesza M, Bzowska M, Wyroba B, Kilarski WW, Bereta J
  TITLE     Overcoming inefficient secretion of recombinant VEGF-C in 
            baculovirus expression vector system by simple purification of the 
            protein from cell lysate.
  JOURNAL   Protein Expr Purif. 2015 Mar 7. pii: S1046-5928(15)00040-6. doi: 
            10.1016/j.pep.2015.03.001.
  PUBMED    25758709
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif. 2015 Mar 7. pii: S1046-5928(15)00040-6. doi: 
            10.1016/j.pep.2015.03.001."
COMMENT     Sequence Label: HBM-mVEGF-C-HisTag-pENTR
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..58
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     160..176
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     160..176
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     181..200
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        181..199
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    217..316
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(342..359)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     sig_peptide     complement(720..782)
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
                     /translation="MKFLVNVALVFMVVYISYIYA"
ORIGIN
        1 ttggaattta atcgcggcct cgacgtttcc cgttgaatat ggctcataac accccttgta
       61 ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca
      121 atgtaacatc agagattttg agacacgggc cagagctgcc aggaaacagc tatgaccatg
      181 taatacgact cactataggg gatatcagct ggatggcaaa taatgatttt attttgactg
      241 atagtgacct gttcgttgca acaaattgat aagcaatgct ttcttataat gccaactttg
      301 tacaagaaag ctgggtcggc gcgcccaccc ttttaaattc agtggtggtg gtggtggtgc
      361 tcgagacgtc taataattga atgaacttgt ctgtaaacat ccagtttaga catgcaccgg
      421 caggaagtgt gattggcaaa actgattgtg actggtttgg ggccttgtga gagaggcact
      481 gtaatttcaa acaacgtctt gctgaggtaa cctgtgctgg tgttcatgca ctgcagcccc
      541 tcgctgttgc agcaaccccc acatctgtag acggacacac atggaggttt aaagaaggtg
      601 tttgtggctg ctccaaactc cttccccaca tctatacaca cctcacgtgg catgcattga
      661 gtctttctcc actcattatc aatacttttc aggatctctg tgttataatg tgcggatccg
      721 gcatagatgt aagaaatgta tacgaccata aaaacaaggg caacgttgac taagaatttc
      781 atggtgaagg gggcggccgc ggagcctgct tt
//
