LOCUS       sequence-110610-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Macrolide biosensor. High copy plasmid that expresses GFP 
            proportional to macrolide concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-H-mphR
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(469..555)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     708..725
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 tgtggtccgg gtaacgagcg aagcactgaa caccgtaggt cagggtggta accagggtcg
       61 gccacggaac cggcagttta ccggtggtgc agatgaattt cagggtcagt ttaccgttgg
      121 tagcgtcacc ttcaccttca ccacgaacag agaatttgtg accgttaacg tcaccgtcca
      181 gttcaaccag gatcggaaca acaccggtga acagttcttc acctttacgc atatgtatat
      241 ctccttctta aagttaaatg ttgacgggtt tagccaccta aatgtaacag tcacgtcggt
      301 tatattcaat ccgtcggcct tacttgctag cagagtttgt agaaacgcaa aaaggccatc
      361 ctgacggatg gccttctgct taatttgatg cctggcagtt tatggcgggc gtcctgcccg
      421 ccaccctccg ggccgttgct tcgcaacgtt caaatccgct cccggcggat ttgtcctact
      481 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc
      541 ctttcgtttt atttgatgcc tggcagttcc ctactctcgc atggggagac cccacactac
      601 catcggcgct acggcgtttc acttctgagt tcggcatggg gtcaggtggg accaccgcgc
      661 tactgccgcc aggcaaattc tgttttatca gaccgcttct gcgttctgat ttaatctgta
      721 tcaggctgaa aatcttctct catccgccaa aacagcctga gcaaaaggcc agcaaaaggc
      781 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      841 g
//
