LOCUS       sequence-110617-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Creating Gal80 lines from MiMIC lines with inserts into coding 
            introns in Phase 0. Gal80 is tagged with FLAG, HA and EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pBS-KS-attB2-SA(0)-T2A-3X Gal80-Hsp70
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    48..117
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             284..337
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 actcactata gggcgaattg gagctcgcta gcgatgtagg tcacggtctc gaagccgcgg
       61 tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccacctc acccatctgg
      121 tccatcatga tggtcgactc tagaagtcga tccaacatgg cgacttgtcc catccccggc
      181 atgtttaaat atactaatta ttcttgaact aattttaatc aaccgattta tctctcttcc
      241 gcaggtggga ggttccggtg gaagcggagg tagcggcgga tccgagggcc gcggcagcct
      301 gctgacctgc ggcgatgtgg aggagaaccc cgggcccatg gattacaaca aaaggagtag
      361 tgtgagtacg gtgccgaatg ctgctcccat tcgcgtgggc ttcgtgggat tgaacgcggc
      421 taagggttgg gccattaaaa cgcattatcc agccatactg cagctgagct cccagttcca
      481 aataacagcg ctttattccc ccaagatcga gacgtccatt gcgacaattc agaggctgaa
      541 gttgtccaac gccacagctt tcccaacact cgagagcttc gcctcgagct ccacgatcga
      601 tatgattgtg atcgcaatcc aggtggcttc ccactacgag gtggtaatgc ccctgctcga
      661 gttttcgaag aacaatccta atctgaagta tttgtttgtg gaatgggcac tggcctgctc
      721 gctcgatcag gcggagtcga tctataaggc cgctgcagag cggggcgtgc aaacaataat
      781 cagtctgcag ggacgaaaga gcccgtacat tttgcgggca
//
