LOCUS       sequence-110621-         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Creating Gal80 lines from MiMIC lines with inserts into coding 
            introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pBS-KS-attB2-SA(2)-T2A-3X Gal80-Hsp70
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    48..117
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             285..338
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 actcactata gggcgaattg gagctcgcta gcgatgtagg tcacggtctc gaagccgcgg
       61 tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccacctc acccatctgg
      121 tccatcatga tggtcgactc tagaagtcga tccaacatgg cgacttgtcc catccccggc
      181 atgtttaaat atactaatta ttcttgaact aattttaatc aaccgattta tctctcttcc
      241 gcaggtcggg aggttccggt ggaagcggag gtagcggcgg atccgagggc cgcggcagcc
      301 tgctgacctg cggcgatgtg gaggagaacc ccgggcccat ggattacaac aaaaggagta
      361 gtgtgagtac ggtgccgaat gctgctccca ttcgcgtggg cttcgtggga ttgaacgcgg
      421 ctaagggttg ggccattaaa acgcattatc cagccatact gcagctgagc tcccagttcc
      481 aaataacagc gctttattcc cccaagatcg agacgtccat tgcgacaatt cagaggctga
      541 agttgtccaa cgccacagct ttcccaacac tcgagagctt cgcctcgagc tccacgatcg
      601 atatgattgt gatcgcaatc caggtggctt cccactacga ggtggtaatg cccctgctcg
      661 agttttcgaa gaacaatcct aatctgaagt atttgtttgt ggaatgggca ctggcctgct
      721 cgctcgatca ggcggagtcg atctataagg ccgctgcaga gcggggcgtg caaacaataa
      781 tcagtctgca gggacgaaag agcccgtaca ttttgcgggc aaaagagctt atctcccagg
      841 gctacatcgg cgatataaat agcatcgaga ttgcaggtaa cggaggttgg tacggttac
//
