LOCUS       sequence-110622-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: myr-FLAG-AKT1-pcw107
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             85..126
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             151..174
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    181..205
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcaccgaacc tctctcccca ggggctagca tcgatggatc aacaagtttt gtacaaaaaa
       61 gttggcgagg gcggcggcgg caccatgggg tcttcaaaat ctaaaccaaa ggaccccagc
      121 cagcgccggc gcaggatccg aggttacctt gactacaaag acgatgacga caagcaattg
      181 acaagtttgt acaaaaaagt tggcatgagc gacgtggcta ttgtgaagga gggttggctg
      241 cacaaacgag gggagtacat caagacctgg cggccacgct acttcctcct caagaatgat
      301 ggcaccttca ttggctacaa ggagcggccg caggatgtgg accaacgtga ggctcccctc
      361 aacaacttct ctgtggcgca gtgccagctg atgaagacgg agcggccccg gcccaacacc
      421 ttcatcatcc gctgcctgca gtggaccact gtcatcgaac gcaccttcca tgtggagact
      481 cctgaggagc gggaggagtg gacaaccgcc atccagactg tggctgacgg cctcaagaag
      541 caggaggagg aggagatgga cttccggtcg ggctcaccca gtgacaactc aggggctgaa
      601 gagatggagg tgtccctggc caagcccaag caccgcgtga ccatgaacga gtttgagtac
      661 ctgaagctgc tgggcaaggg cactttcggc aaggtgatcc tggtgaagga gaaggccaca
      721 ggccgctact acgccatgaa gatcctc
//
