LOCUS       sequence-110623-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tetracycline biosensor. Low copy plasmid that expresses GFP 
            proportional to tetracycline concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-L-tetR
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    296..314
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    321..339
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(501..587)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     740..757
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 ggtccgggta acgagcgaag cactgaacac cgtaggtcag ggtggtaacc agggtcggcc
       61 acggaaccgg cagtttaccg gtggtgcaga tgaatttcag ggtcagttta ccgttggtag
      121 cgtcaccttc accttcacca cgaacagaga atttgtgacc gttaacgtca ccgtccagtt
      181 caaccaggat cggaacaaca ccggtgaaca gttcttcacc tttacgcata ttatatctcc
      241 ttcttaaagt taaatttaat gaattcggtc atgcgtcctg ctgatgtgct cagtatctct
      301 atcactgata gggatgtcaa tctctatcac tgatagggac tcgagtcggc cttacttgct
      361 agcagagttt gtagaaacgc aaaaaggcca tcctgacgga tggccttctg cttaatttga
      421 tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcgcaacg
      481 ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag
      541 ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctggcagtt
      601 ccctactctc gcatggggag accccacact accatcggcg ctacggcgtt tcacttctga
      661 gttcggcatg gggtcaggtg ggaccaccgc gctactgccg ccaggcaaat tctgttttat
      721 cagaccgctt ctgcgttctg atttaatctg tatcaggctg aaaatcttct ctcatccgcc
      781 aaaacagcca gttgatgata ccgctgcctt actgggtgca ttagccagtc tgaa
//
