LOCUS       sequence-110626-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Creating dVP16AD hemidrivers from MiMIC lines with inserts into 
            coding introns in Phase 0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pBS-KS-attB2-SA(0)-T2A-p65AD-Hsp70
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    46..115
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             282..335
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             366..386
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcactatagg gcgaattgga gctcgctagc gatgtaggtc acggtctcga agccgcggtg
       61 cgggtgccag ggcgtgccct tgggctcccc gggcgcgtac tccacctcac ccatctggtc
      121 catcatgatg gtcgactcta gaagtcgatc caacatggcg acttgtccca tccccggcat
      181 gtttaaatat actaattatt cttgaactaa ttttaatcaa ccgatttatc tctcttccgc
      241 aggtgggagg ttccggtgga agcggaggta gcggcggatc cgagggccgc ggcagcctgc
      301 tgacctgcgg cgatgtggag gagaaccccg ggcccatgga taaagcggaa ttaattcccg
      361 agcctccaaa aaagaagaga aaggtcgaat tgggttccac gccgatggag ttccagtacc
      421 tgcccgatac ggatgaccgt caccgtatcg aagaaaagcg gaagcgaacc tatgaaacct
      481 tcaagtccat catgaaaaag tcccccttct cgggccccac ggacccgcgc cccccgcccc
      541 gtcgtattgc ggttccttcg cgcagcagtg ccagcgtccc caaacccgca ccgcagccct
      601 acccgttcac ttcctccctt agcacgatta actatgatga gttccccacg atggtgttcc
      661 ccagtggaca aatttcccag gcatcggcac tggctccggc cccaccgcaa gtcctccccc
      721 aggcgcccgc tccggcaccg gctcccgcaa tggtgagtgc tctggcccag gcccccgctc
      781 cagtccccgt gctggcgcct ggacccccac aggcagttgc ccctcctgct ccgaaaccaa
      841 cgcaggcggg cgaaggaacc ctg
//
