LOCUS       sequence-110637-         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recipient integrative vector to construct FLAG tagged fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Yanez-Carrillo P, Orta-Zavalza E, Gutierrez-Escobedo G, 
            Patron-Soberano A, De Las Penas A, Castano I
  TITLE     Expression vectors for C-terminal fusions with fluorescent proteins 
            and epitope tags in Candida glabrata.
  JOURNAL   Fungal Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020.
  PUBMED    25986172
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020."
COMMENT     Sequence Label: pYC46
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..51
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     35..51
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             61..84
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             91..114
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             121..144
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(156..189)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gggcgaattg gagctccacc gcggtggcgg ccgctctaga actagtggat cctaggagca
       61 gactacaaag atgacgatga caaaggagcc gattataagg acgatgacga taaaggggct
      121 gactacaaag atgacgatga caagtgataa gatccgaagt tcctatactt tctagagaat
      181 aggaacttcg tgcgcttttg aaccacgtaa agtgctgtaa accagctcta ttcaatgagg
      241 taaatgataa agtttataag agaccaaaaa attcaaaaaa aaaattgaaa aattatattg
      301 gtatttatat gaaacaatag ttcagttcaa agcgctagct tttctcatct caactaaccg
      361 gtattaggtg ggtatttatt taatatggga acaagaaatg tgacattaat gttcatgttt
      421 ttattttcat tatttattct atatggtttg ggacttgatg ttatgtctta attcatagag
      481 atccgcttgc ctcgtccccg ccgggtcacc cggccagcga catggaggcc cagaataccc
      541 tccttgacag tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc ccgtacattt
      601 agcccataca tccccatgta taatcatttg catccataca ttttgatggc cgcacggcgc
      661 gaagcaaa
//
