LOCUS       sequence-110652-         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express sgNanog in mammalian cells of mice..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Yu C, Liu Y, Ma T, Liu K, Xu S, Zhang Y, Liu H, La Russa M, Xie M, 
            Ding S, Qi LS
  TITLE     Small Molecules Enhance CRISPR Genome Editing in Pluripotent Stem 
            Cells.
  JOURNAL   Cell Stem Cell. 2015 Feb 5;16(2):142-7. doi: 
            10.1016/j.stem.2015.01.003.
  PUBMED    25658371
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2015.01"; journalName: "Cell Stem Cell"; date: 
            "2015-02-5- 5"; volume: "16"; issue: "2"; pages: "142-7"
COMMENT     Sequence Label: pSLQ1371_sgNanog
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(117..137)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gcatgttagc agacttcctc tgccctctcc cgatccggca ccgggcttgc gggtcatgca
       61 ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc cgagccgctc
      121 gtagaagggg aggttgcggg gcgcggaggt ctccaggaag gcgggcaccc cggcgcgctc
      181 ggccgcctcc actccgggga gcacgacggc gctgcccaga cccttgccct ggtggtcggg
      241 cgagacgccg acggtggcca ggaaccacgc gggctccttg ggccggtgcg gcgccaggag
      301 gccttccatc tgttgctgcg cggccagccg ggaaccgctc aactcggcca tgcgcgggcc
      361 gatctcggcg aacaccgccc ccgcttcgac gctctccggc gtggtccaga ccgccaccgc
      421 ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg tgaggaagag
      481 ttcttgcagc tcggtgaccc gctcgatgtg gcggtccggg tcgacggtgt ggcgcgtggc
      541 ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc cgggggacgt cgtcgcgggt
      601 ggcgaggcgc accgt
//
