LOCUS       sequence-110668-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for murine Fc-FLAG, used for monomeric 
            Fc-IL2 production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Zhu EF, Gai SA, Opel CF, Kwan BH, Surana R, Mihm MC, Kauke MJ, 
            Moynihan KD, Angelini A, Williams RT, Stephan MT, Kim JS, Yaffe MB, 
            Irvine DJ, Weiner LM, Dranoff G, Wittrup KD
  TITLE     Synergistic Innate and Adaptive Immune Response to Combination 
            Immunotherapy with Anti-Tumor Antigen Antibodies and Extended Serum 
            Half-Life IL-2.
  JOURNAL   Cancer Cell. 2015 Apr 13;27(4):489-501. doi: 
            10.1016/j.ccell.2015.03.004.
  PUBMED    25873172
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2015.03"; journalName: "Cancer Cell"; date: 
            "2015-04-13- 13"; volume: "27"; issue: "4"; pages: "489-501"
COMMENT     Sequence Label: FcD265A-FLAG
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             787..810
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgagggtcc ccgctcagct cctggggctc ctgctgctct ggctcccagg tgcacgatgt
       61 gagcccagag tgcccataac acagaacccc tgtcctccac tcaaagagtg tcccccatgc
      121 gcagctccag acctcttggg tggaccatcc gtcttcatct tccctccaaa gatcaaggat
      181 gtactcatga tctccctgag ccccatggtc acatgtgtgg tggtggccgt gagcgaggat
      241 gacccagacg tccagatcag ctggtttgtg aacaacgtgg aagtacacac agctcagaca
      301 caaacccata gagaggatta caacagtact ctccgggtgg tcagtgccct ccccatccag
      361 caccaggact ggatgagtgg caaggagttc aaatgcaagg tcaacaacag agccctccca
      421 tcccccatcg agaaaaccat ctcaaaaccc agagggccag taagagctcc acaggtatat
      481 gtcttgcctc caccagcaga agagatgact aagaaagagt tcagtctgac ctgcatgatc
      541 acaggcttct tacctgccga aattgctgtg gactggacca gcaatgggcg tacagagcaa
      601 aactacaaga acaccgcaac agtcctggac tctgatggtt cttacttcat gtacagcaag
      661 ctcagagtac aaaagagcac ttgggaaaga ggaagtcttt tcgcctgctc agtggtccac
      721 gagggtctgc acaatcacct tacgactaag accatctccc ggtctctggg taaaggtggc
      781 ggatctgact acaaggacga cgatgacaag tgataa
//
