LOCUS       sequence-110669-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flavonoid biosensor. High copy plasmid that expresses GFP 
            proportional to flavonoid concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-H-ttgR
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(347..374)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(466..552)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     705..722
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 tggtccgggt aacgagcgaa gcactgaaca ccgtaggtca gggtggtaac cagggtcggc
       61 cacggaaccg gcagtttacc ggtggtgcag atgaatttca gggtcagttt accgttggta
      121 gcgtcacctt caccttcacc acgaacagag aatttgtgac cgttaacgtc accgtccagt
      181 tcaaccagga tcggaacaac accggtgaac agttcttcac ctttacgcat atgtatatct
      241 ccttcttaaa gttaaatgtt gctaaggaat atacttacat tcatggttgt ttgtaaatac
      301 tgctgggtgg tcggccttac ttgctagcag agtttgtaga aacgcaaaaa ggccatccgt
      361 caggatggcc ttctgcttaa tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
      421 ccctccgggc cgttgcttcg caacgttcaa atccgctccc ggcggatttg tcctactcag
      481 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt
      541 tcgttttatt tgatgcctgg cagttcccta ctctcgcatg gggagacccc acactaccat
      601 cggcgctacg gcgtttcact tctgagttcg gcatggggtc aggtgggacc accgcgctac
      661 tgccgccagg caaattctgt tttatcagac cgcttctgcg ttctgattta atctgtatca
      721 ggctgaaaat cttctctcat ccgccaaaac agccatgagc aaaaggccag caaaaggcca
      781 ggaaccgt
//
