LOCUS       sequence-110673-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flavonoid biosensor. Low copy plasmid that expresses GFP 
            proportional to flavonoid concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-L-ttgR
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(285..312)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(404..490)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     643..660
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 cggaaccggc agtttaccgg tggtgcagat gaatttcagg gtcagtttac cgttggtagc
       61 gtcaccttca ccttcaccac gaacagagaa tttgtgaccg ttaacgtcac cgtccagttc
      121 aaccaggatc ggaacaacac cggtgaacag ttcttcacct ttacgcatat gtatatctcc
      181 ttcttaaagt taaatgttgc taaggaatat acttacattc atggttgttt gtaaatactg
      241 ctgggtggtc ggccttactt gctagcagag tttgtagaaa cgcaaaaagg ccatccgtca
      301 ggatggcctt ctgcttaatt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
      361 ctccgggccg ttgcttcgca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
      421 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc
      481 gttttatttg atgcctggca gttccctact ctcgcatggg gagaccccac actaccatcg
      541 gcgctacggc gtttcacttc tgagttcggc atggggtcag gtgggaccac cgcgctactg
      601 ccgccaggca aattctgttt tatcagaccg cttctgcgtt ctgatttaat ctgtatcagg
      661 ctgaaaatct tctctcatcc gccaaaacag ccagttgatg ataccgctgc cttactgggt
//
