LOCUS       sequence-110675-         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: beta-catenin (S33A, S37A, T41A, S45A)-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..65
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             128..163
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 tcaccgacct ctctccccag gggctagcat cgatggatca acaagtttgt acaaaaaagt
       61 tggcaaatgc ggcggcggca ccatggctac tcaagctgac ctgatggagt tggacatggc
      121 catggagccg gacagaaaag ctgctgtcag ccactggcag cagcagtctt acttggatgc
      181 tggaatccat gctggtgcca ccgccacagc tcctgccctg agtggcaagg gcaaccctga
      241 ggaagaagat gttgacacct cccaagtcct ttatgaatgg gagcaaggct tttcccagtc
      301 cttcacgcaa gagcaagtag ctgatattga cgggcagtat gcaatgacta gggctcagag
      361 ggtccgagct gccatgttcc ctgagacgct agatgagggc atgcagatcc catccacgca
      421 gtttgacgct gctcatccca ctaatgtcca gcgcttggct gaaccatcac agatgttgaa
      481 acatgcagtt gtcaatttga ttaactatca ggatgacgcg gaacttgcca cacgtgcaat
      541 tcctgagctg acaaaactgc taaacgatga ggaccaggtg gtagttaata aagctgctgt
      601 tatggtccat cagctttcca aaaaggaagc ttccagacat gccatcatgc gctcccctca
      661 gatggtgtct gccattgtac gcaccatgca g
//
